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Showing results 1 to 5 of 5
  • OER000002511.pdf.jpg
  • Journal article

  • Biophysical characterization and analysis of a mesophilic chorismate mutase from B. pumilus

    • Authors : Wilkins, Ryan Scott  (2023)

  • Chorismate mutases have extensively been used as computational benchmarking systems for enzyme catalysis, yet the roles entropy and enthalpy play in catalysis are still not fully understood. Thus, it is important to better understand these enzymes for potential research or industrial applications. Here, we report the first crystal structure and kinetic characterization of a chorismate mutase from Bacillus pumilus. This enzyme exhibits a high degree of similar...
  • OER000002397.pdf.jpg
  • Journal Article

  • Centrifugal Gel Crushing for Gel-based Proteome Analysis

    • Authors : Nishida, Hiroshi (2023)

  • We have developed a centrifugal gel crushing method using a pipette tip. Polyacrylamide gel slices are extruded from the narrowing cavity of a pipette tip by centrifugation in a few minutes to crush them into pieces of appropriate size. The size of the crushed gel could be controlled by several parameters, including centrifugal force and pipette tip cavity. In shotgun proteomics, gel-based LC/MS/MS, so-called GeLC/MS/MS, involves the ...
  • OER000002364.pdf.jpg
  • Journal Article

  • Drug repurposing approach for potential Pfmrk inhibitors as antimalarial agents: an In-silico analysis

    • Authors : McBride, John M. (2023)

  • Alteration of one or few amino acid residues can affect structure [1–3] and function [4] of a protein and, in extreme cases, be the difference between health and disease [5, 6]. Understanding structural consequences of point mutations is important for drug design [7, 8] and could also accelerate optimization of enzymatic function via directed evolution [9, 10]. In these and other applications, theoretical models [11] could...
  • OER000002291.pdf.jpg
  • Journal Article

  • Identification of putative druggable pockets in PRL3, a significant oncology target, using in silico analysis

    • Authors : Bennett, Grace M. (2023)

  • Aberrant cellular phosphorylation is a hallmark of several diseases including inflammation and cancers (1–3). Protein phosphorylation is a post-translational modification that can act as a switch to regulate biochemical pathways and is regulated by the concerted action of two classes of enzymes: kinases and phosphatases. As such, kinases and phosphatases present as significant potential clinical molecular targets. To date, several kinase inhibitors have&#...
  • OER000002984.pdf.jpg
  • Journal article

  • Simplified high-throughput methods for deep proteome analysis on the timsTOF Pro

    • Authors : Sandow, Jarrod J (2021)

  • Recent advances in mass spectrometry technology have seen remarkable increases in proteomic sequencing speed, while improvements to dynamic range have remained limited. An exemplar of this is the new timsTOF Pro instrument, which thanks to its trapped ion mobility, pushes effective fragmentation rates beyond 100Hz and provides accurate CCS values as well as impressive sensitivity. Established data dependent methodologies underutilize&...