- Journal article
Authors : Tsowa, Swen (2021) - Proteins interacting with ADP-ribosyl groups are often involved in disease-related pathways or in viral
infections, which makes them attractive targets for the development of inhibitors. Our goal was to
develop a robust and accessible assay technology that is suitable for high-throughput screening and
applicable to a wide range of proteins acting as either hydrolysing or non-hydrolysing binders of mono and poly-ADP-ribosyl groups. As a fou...
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- Journal article
Authors : Kwon, Eunju (2023) - AAA+ ATPases are ubiquitous proteins associated with most cellular processes, including DNA 15 unwinding and protein unfolding. Their functional and structural properties are typically 16 determined by domains and motifs added to the conserved ATPases domain. Currently, the 17 molecular function and structure of many ATPases remain elusive. Here, we report the crystal 18 structure and biochemical analyses of YjoB, a Bacillus subtilis AAA+ protein. The crystal...
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- Journal Article
Authors : Schachner, Luis F. (2023) - Glycosylated biotherapeutics are an emerging class of drugs with high molecular
heterogeneity, which can affect their safety and efficacy. Characterizing this
heterogeneity is crucial for drug development and quality assessment, but existing
methods are limited in their ability to analyze intact glycoproteins. Here, we present a
new approach to glycoform fingerprinting that uses proton-transfer charge-reduction
with gas-phase fractionation to analyze intact&#...
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- Journal article
Authors : Lim, Roktaek (2023) - The Michaelis–Menten (MM) rate law has been the dominant paradigm of modeling23
biochemical rate processes for over a century with applications in biochemistry, biophysics,24
cell biology, and chemical engineering. The MM rate law and its remedied form stand on the25
assumption that the concentration of the complex of interacting molecules, at each moment,26
approaches an equilibrium much faster than the molecular concentrations change. Yet, thi...
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- Journal article
Authors : Atang, Alexandra E. (2023) - Spinocerebellar ataxia type 5 (SCA5) is a neurodegenerative disease caused by mutations in the SPTBN2 gene encoding the cytoskeletal protein β-III-spectrin. Previously, we demonstrated that a L253P missense mutation, localizing to the β-III-spectrin actin-binding domain (ABD), causes increased actin-binding affinity. Here we investigate the molecular consequences of nine additional ABD-localized, SCA5 missense mutations: V58M, K61E, T62I, K65E, F160C, D255G, T271I, Y272H, and H2...
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- Journal Article
Authors : Thore, Stéphane (2023) - Eukaryotic pre-mRNA is processed by a large multiprotein complex to accurately cleave the 3’ end, and to catalyze the addition of the poly(A) tail. Within this cleavage and polyadenylation specificity factor (CPSF) machinery, the CPSF73 endonuclease subunit directly contacts both CPSF100 and the scaffold protein Symplekin to form a subcomplex known as the core cleavage complex (CCC) or mammalian cleavage factor (mCF). Here we have taken advantage of ...
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- Journal article
Authors : Halpin, Jackson C. (2023) - TRAF6 is an adapter protein and E3 ubiquitin ligase involved in signaling downstream of cell receptors essential for development and the immune system. TRAF6 participates in many protein-protein interactions, some of which are mediated by a C-terminal MATH domain that recruits TRAF6 to cell-surface receptors and associated proteins. The TRAF6 MATH domain binds to short peptide segments containing the motif PxExx[FYWHDE], where x is any amino acid. Blocki...
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- Journal Article
Authors : Maharana, Jagannath (2023) - β-arrestins are multifunctional proteins that are critically involved in regulating spatio26
temporal aspects of GPCR signaling. The interaction of β-arrestins with GPCRs is typically
conceptualized in terms of receptor activation and phosphorylation primarily in the carboxyl
terminus. Interestingly however, there are several GPCRs that harbor majority of
phosphorylation sites in their 3rd intracellular loop (ICL3) instead of carboxyl-terminus but still
...
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- Journal article
Authors : Perdikari, Theodora Myrto (2023) - Numerous cell biology studies have used high concentrations of 1,6-hexanediol to dissolve
membraneless organelles and disordered protein biomolecular condensates. Yet, little is known
about how alkanediols effect liquid-liquid phase separation (LLPS), and why certain alkanediol
isomers are more effective. Here, we evaluate the effect of various alkanediols on the
archetypal phase separating protein FUS. Low-complexity domain and full-length FUS LLPS is
d...
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- Journal article
Authors : Oosterheert, Wout (2023) - The release of inorganic phosphate (Pi) from actin filaments constitutes a key step in their
regulated turnover, which is fundamental to many cellular functions. However, the molecular
mechanisms underlying Pi release from both the core and barbed end of actin filaments remain
unclear. Here, we combine cryo-EM with molecular dynamics simulations and in vitro
reconstitution to demonstrate how actin releases Pi through a ‘molecular backdoor’. ...
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- Ebooks (Sách điện tử)
Authors : Christopher (2023) - Immunomodulatory drugs (IMiDs), which include
thalidomide and its derivatives, have emerged as the standard of care
against multiple myeloma. They function as molecular glues that bind
to the E3 ligase cereblon (CRBN) and induce protein interactions with
neosubstrates, including the transcription factors Ikaros (IKZF1) and
Aiolos (IKZF3). The subsequent ubiquitylation and degradation of
these transcription factors underlies the antiproliferative activity...
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