- Journal article
Authors : George, Amy L. (2023) - Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target and off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due to target engagement requires high quantitative accuracy and consistent detection. Isobaric tandem mass tags (TMT) are used to multiplex samples and increase quantification precision in TPP analysis by data-dependent acqui...
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- Journal article
Authors : Gerasimaitė, Rūta (2023) - The development of live-cell fluorescence nanoscopy is powered by the availability of suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling intracellular structures. Isomeric tuning is a powerful method for optimizing biocompatibility of the rhodamine-containing probes without affecting their spectral properties. However, the efficient synthesis pathway for rhodamine 4-isomers is still lacking. Herein, we present a facile protecting-group-free 4-carboxyrhoda...
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- Journal article
Authors : Morgan, Rhiannon S. (2021) - The embryonic zebrafish is an ideal system for lipid analyses with relevance to many areas of
bioscience research, including biomarkers and therapeutics. Research in this area has been hampered
by difficulties in extracting, identifying and quantifying lipids. We employed 1H-NMR at 700MHz to profile lipids in developing zebrafish embryos. The optimal method for lipidomics in embryonic zebrafish incorporated rapid lipid extraction using chlorof...
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- Journal article
Authors : Judith, Simon (2020) - Recently, amid growing awareness that computer algorithms are not neutral tools but can
cause harm by reproducing and amplifying bias, attempts to detect and prevent such biases have
intensified. An approach that has received considerable attention in this regard is the Value
Sensitive Design (VSD) methodology, which aims to contribute to both the critical analysis of
(dis)values in existing technologies and the construction o...
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- Journal article
Authors : Christensen, Idd A. (2023) - Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze oxidative cleavage of polysaccharides, such as cellulose and chitin. LPMO action is key to the efficient varlorization of biomass, but the instability of LPMOs in turnover conditions limits their efficiency. LPMO catalysis requires the presence of a reductant, such as ascorbic acid, and hydrogen peroxide, which can be generated in situ in the presence of molecular oxygen an...
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- Journal article
Authors : Udema, Ikechukwu I. (2023) - By graphical means, the Vmax and KM values for galactosidase respectively range between 163
39 and 185 M/min and between 2.07 and 2.77 mg/L; the range by calculations is 177 and 214 M/min and
40 2.45 and 3.311 mg/L, subject to pseudo-statistical remediation. Overall, the ranges of Vmax and KM values
41 for alpha-amylase from both the graphical method and calculation are, respectively, 1.095 to 1.018
42 mM...
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- Ebooks (Sách điện tử)
Authors : West Coast Editorial Associates (2021) - The purpose of this resource is to provide you with guidance on preparing and revising your own documents through the lens of plain language. By working through one example in detail, it shows you how to keep the focus on the reader, organize the information in order of importance, and use words that most readers will understand. Of course, using plain language is just part of the process. Developing policies and procedures for&#...
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- Journal article
Authors : Sandow, Jarrod J (2021) - Recent advances in mass spectrometry technology have seen remarkable increases in
proteomic sequencing speed, while improvements to dynamic range have remained limited.
An exemplar of this is the new timsTOF Pro instrument, which thanks to its trapped ion
mobility, pushes effective fragmentation rates beyond 100Hz and provides accurate CCS
values as well as impressive sensitivity. Established data dependent methodologies
underutilize&...
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- Journal article
Authors : Nicolay, Worren (2020) - A key task in organization design is to group elements (e.g., roles) into sub-units (e.g.,
teams or departments). This task is computationally challenging as one must take into
consideration a potentially large number of interdependencies between the elements.
It also requires data about work processes in the organization, which are rarely present.
We have initiated a research program that aims at developing a tool—Reconfig—to
improve ...
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- Journal article
Authors : Esch, Bianca M. (2023) - Sphingolipids (SP) are one of the three major lipid classes in eukaryotic cells and serve as38
structural components of the plasma membrane. The rate-limiting step in SP biosynthesis is39
catalyzed by serine palmitoyltransferase (SPT). In yeast, SPT consists of two catalytic subunits40
(Lcb1 and Lcb2), a regulatory subunit (Tsc3), negative regulators (Orm1 and Orm2), and the41
phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1, collectively known as...
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