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  • OER000002943.pdf.jpg
  • Journal article

  • Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS

    • Authors : Vaňková, Zuzana (2023)

  • Reversed-phase ultrahigh-performance liquid chromatography – mass spectrometry (RP-UHPLC/MS) method was developed with the aim to unambiguously identify a large number of lipid species from multiple lipid classes in human plasma. The optimized RP-UHPLC/MS method employed the C18 column with sub-2 μm particles with the total run time of 25 min. Thechromatographic resolution was investigated with 42 standards from 18 lipid classes. The UHPLC system was cou...