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Showing results 1 to 5 of 5
  • OER000002940.pdf.jpg
  • Journal article

  • Cell-free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs

    • Authors : Do, Hoa Quynh (2021)

  • The Proton-Coupled Folate Transporter (PCFT) is a transmembrane transport protein that controls the absorption of dietary folates in the small intestine. PCFT also mediates uptake of chemotherapeutically used antifolates into tumor cells. PCFT has been identified within lipid rafts observed in phospholipid bilayers of plasma membranes, a micro environment that is altered in tumor cells. The present study aimed at investigating the ...
  • OER000002769.pdf.jpg
  • Journal article

  • Cotranslational folding and assembly of the dimeric E. coli inner membrane protein EmrE

    • Authors : Mermans, Daphne (2023)

  • In recent years, it has become clear that many homo- and heterodimeric cytoplasmic proteins in both prokaryotic and eukaryotic cells start to dimerize cotranslationally, i.e., while at least one of the two chains is still attached to the ribosome. Whether this is possible also for integral membrane proteins is unknown, however. Here, we apply Force Profile Analysis (FPA) – a method where a translational arrest peptide (AP) enginee...
  • OER000002472.pdf.jpg
  • Journal Article

  • Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers

    • Authors : Krishnarjuna, Bankala (2023)

  • The detergent-free isolation of membrane proteins using synthetic polymers is becoming the desired approach for functional and structural studies of membrane proteins. Since the expression levels for many membrane proteins are low and a high yield of functionalized reconstituted membrane proteins is essential for in vitro studies, it is crucial to optimize the experimental conditions for a given polymer to effectively solubilize target membranes/...
  • OER000003007.pdf.jpg
  • Journal article

  • Identification and assessment of cardiolipin interactions with E. coli inner membrane proteins

    • Authors : Corey, Robin A. (2021)

  • Integral membrane proteins are localised and/or regulated by lipids present in the surrounding bilayer. Whilst bacteria such as E. coli have relatively simple membranes when compared to eukaryotic cells, there is ample evidence that many bacterial proteins bind to specific lipids, especially the anionic lipid cardiolipin. Here, we apply molecular dynamics simulations to assess lipid binding to 42 different E. coli inner membrane proteins. O...
  • OER000002429.pdf.jpg
  • Ebooks (Sách điện tử)

  • Large-scale column-free purification of bovine F-ATP synthase

    • Authors : Chimari, Jiko (2023)

  • Mammalian F-ATP synthase is central to mitochondrial bioenergetics and is present in the inner mitochondrial membrane in a dynamic oligomeric state of higher oligomers, tetramers, dimers and monomers. In vitro investigations of mammalian F-ATP synthase are often limited by the ability to purify the oligomeric forms present in vivo at sufficient quantity, stability and purity. We developed a purification approach for the isolation of bovine ...