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Browsing by Subject ribosome

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Showing results 1 to 5 of 5
  • OER000002951.pdf.jpg
  • Journal article

  • A comprehensive landscape of 60S ribosome biogenesis factors

    • Authors : Sailer, Carolin (2023)

  • Eukaryotic ribosome biogenesis is facilitated and regulated by numerous ribosome biogenesis factors (RBFs). High-resolution cryo-EM maps have defined the molecular interactions of RBFs during maturation, but many transient and dynamic interactions, particularly during early assembly, remain uncharacterized. Using quantitative proteomics and crosslinking coupled to mass spectrometry (XL-MS) data from a extensive set of pre-ribosomal particles, we derived a comprehensive...
  • OER000002757.pdf.jpg
  • Journal article

  • Clearing of ribosome collisions by the ribosome quality control trigger complex RQT

    • Authors : Best, Katharina (2023)

  • After translational stalls, colliding eukaryotic ribosomes are cleared through dissociation into subunits by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryo-EM of several RQT-ribosome complexes revealed the structural basis of splitting: RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose...
  • OER000002917.pdf.jpg
  • Journal article

  • Mechanistic studies of non-canonical amino acid mutagenesis

    • Authors : Fleisher, Rachel C. (2021)

  • Over the past decade, harnessing the cellular protein synthesis machinery to incorporate non canonical amino acids (ncAAs) into tailor-made peptides has significantly advanced many aspects of molecular science. More recently, groundbreaking progress in our ability to engineer this machinery for improved ncAA incorporation has led to significant enhancements of this powerful tool for biology and chemistry. By revealing the molecular basis for t...
  • OER000002695.pdf.jpg
  • Journal article

  • Methylated guanosine and uridine modifications in S. cerevisiae mRNAs modulate translation elongation

    • Authors : Jones, Joshua D. (2023)

  • Chemical modifications to protein encoding messenger RNA (mRNA) can modulate their localization, translation and stability within cells. Over 15 different types of mRNA modifications have been identified by sequencing and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) technologies. While LC-MS/MS is arguably the most essential tool available for studying analogous protein post-translational modifications, the high-throughput discovery and quantitative characteri...