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Journal article

A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening

Authors : - (2023)

Functional screening of environmental DNA (eDNA) libraries is a potentially powerful approach to2 discover enzymatic “unknown unknowns”, but is usually heavily biased toward the tiny subset of3 genes preferentially transcribed and translated by the screening strain. We have overcome this by4 preparing an eDNA library via partial digest with restriction enzyme FatI (cuts CATG), causing a5 substantial proportion of ATG start codons to be precisely...