- Journal article
Authors : Piper, Isabel M. (2021) - Gram-positive bacteria contain sortase enzymes on their cell surfaces that catalyze transpeptidation
reactions critical for proper cellular function. In vitro, sortases are used in sortase-mediated ligation (SML)
reactions for a variety of protein engineering applications. Historically, sortase A from Staphylococcus
aureus (saSrtA) has been the enzyme of choice for SML reactions. However, the stringent specificity of
saSrtA for the sequence motif LPXTG li...
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- Journal article
Authors : Lipinski, Karli A. (2023) - Precursor mRNA (pre-mRNA) splicing is an essential process for gene expression in
15 eukaryotes catalyzed by the spliceosome in two transesterification steps. The spliceosome is a
16 large, highly dynamic complex composed of 5 small nuclear RNAs and dozens of proteins, some
17 of which are needed throughout the splicing reaction while others only act during specific stages.
18 The human protein FAM192A was recently proposed to be&...
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- Journal article
Authors : Yadav, Ruchi (2023) - SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19
34 pandemic. Its ~30 kb RNA genome is translated into two large polyproteins subsequently cleaved
35 by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein processing is essential
36 yet incompletely understood. We studied Mpro-mediated processing of the nsp7-10/11
37 polyprotein, whose mature products are cofactors of the viral replicase, identifying the&#...
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- Journal article
Authors : Bihani, Ashish (2023) - Nuclear Matrix (NuMat) is the fraction of the eukaryotic nucleus insoluble to detergents and
high-salt extractions that manifests as a pan-nuclear fiber-granule network. NuMat consists of
ribonucleoprotein complexes, members of crucial nuclear functional modules, and DNA
fragments. Although NuMat captures the organization of non-chromatin nuclear space, very little
is known about component organization within NuMat. To understand the organization of
NuMat...
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- Journal article
Authors : Harris, Noah J (2021) - Transport Protein Particle complexes (TRAPP) are evolutionarily conserved regulators of membrane trafficking, with this mediated by their guanine nucleotide exchange factor (GEF) activity towards Rab GTPases. In metazoans evidence suggests that two different TRAPP complexes exist, TRAPPII and TRAPPIII. These two complexes share a common core of subunits, with complex specific subunits (TRAPPC9 and TRAPPC10 in TRAPPII and TRAPPC8, TRAPPC11, TRAPPC12, TRAP...
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- Journal article
Authors : Cueny, Rachel R. (2023) - Bloom syndrome helicase (BLM) is a RecQ-family helicase implicated in a variety of cellular
24 processes, including DNA replication, DNA repair, and telomere maintenance. Mutations in
25 human BLM cause Bloom syndrome (BS), an autosomal recessive disorder that leads to myriad
26 negative health impacts including a predisposition to cancer. BS-causing mutations in BLM
27 often negatively impact BLM ATPase and helicase activity. While BLM mut...
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- Journal article
Authors : Kaushik, Lochan (2021) - Guava is a small, tropical fruit tree grown in various tropical and subtropical regions. Salicylic
acid (SA) is a phenolic compound that enhances disease resistance and delays the fruit ripening
process. Calcium is an essential cell component that delays ripening, particularly softening of the
fruit. The effect of foliar spray of CaCl2, and SA, on the physical and biochemical traits of guava
was investigated in the present investi...
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- Journal Article
Authors : bradshaw, william j (2023) - SH2-containing-inositol-5-phosphatases (SHIP1 and SHIP2) are key parts of the PI3K/AKT/mTOR signalling pathway. SHIPs dephosphorylate phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3) to produce phosphatidylinositol-3,4-bisphosphate (PI(3,4)P2). The balance between these two second messengers and related inositol- and phosphatidylinositol phosphates (PIPs) ultimately affects a wide number of cellular processes including inflammation, autophagy, cell migration, metabolism and cell growth.1,2 Therefore, it is&...
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- Journal article
Authors : Lemonidis, Kimon (2023) - The Fanconi Anaemia pathway operates for the repair of interstrand crosslinks and the maintenance
11 of genomic stability upon replication stalling. Di‐monoubiquitination of the FANCI‐FANCD2 (ID2)
12 complex is a central and crucial step in this pathway. Evidence suggests that FANCD2 ubiquitination
13 precedes FANCI ubiquitination, and that both the FANCD2‐ubiquitinated (ID2Ub) and the di14
monoubiquitinated (IUbD2Ub) complex clamp on DNA. However, FANCD2...
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- Journal Article
Authors : Paloyan, Ani (2023) - N-Carbamoyl-β-Alanine Amidohydrolase (CβAA) constitute one of the most important groups of
26 industrially relevant enzymes used in production of optically pure amino acids and derivatives. In
27 this study, a N-carbamoyl-β-alanine amidohydrolase encoding gene from Rhizobium radiobacter
28 MDC 8606 was cloned and overexpressed in Escherichia coli. The purified recombinant enzyme
29 (RrCβAA) showed a specific activity of 14 U/mg using N-carbamoyl- β-a...
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- Journal article
Authors : Selvaraj, Muniyandi (2023) - The actin cytoskeleton is critical for cell migration, morphogenesis, endocytosis, organelle
dynamics, and cytokinesis. To support diverse cellular processes, actin filaments form a
variety of structures with specific architectures and dynamic properties. Key proteins
specifying actin filaments are tropomyosins. Non-muscle cells express several functionally
non-redundant tropomyosin isoforms, which differentially control the interactions of
other proteins, including myosins ...
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- Journal article
Authors : Markus, Linda M. D. (2023) - Cyanophycin is a natural polymer composed of a poly-aspartate backbone with arginine attached
to each of the aspartate sidechains. Produced by a wide range of bacteria, which mainly use it as
a store of fixed nitrogen, it has many promising industrial applications. Cyanophycin can be
synthesized from the amino acids Asp and Arg by the widespread cyanophycin synthetase 1
(CphA1), or from the dipeptide β-Asp-Arg by the cyanobacteria...
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- Journal article
Authors : Jamabo, Miebaka (2021) - African Trypanosomiasis is a neglected tropical disease caused by Trypanosoma brucei (T. brucei) and is spread by the tsetse fly in sub-Saharan Africa. The disease is fatal if left untreated and the currently approved drugs for treatment are toxic and difficult to administer. The trypanosome must survive in the insect vector and its mammalian host, and to adapt to these different conditions, the parasite relies on molecular chaperones called he...
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