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Showing results 1 to 5 of 5
  • OER000002617.pdf.jpg
  • Journal article

  • A Recombinant Approach For Stapled Peptide Discovery Yields Inhibitors of the RAD51 Recombinase

    • Authors : Pantelejevs, Teodors (2023)

  • Stapling is a macrocyclisation method that connects amino acid side chains of a peptide to improve its pharmacological properties. We describe an approach for stapled peptide preparation and biochemical evaluation that combines recombinant expression of fusion constructs of target peptides and cysteine-reactive divinylheteroaryl chemistry, as an alternative to solid-phase synthesis. We then employ this workflow to prepare and evaluate BRC-repeatderived ...
  • OER000002913.pdf.jpg
  • Journal article

  • Characterization of a recombinant plastid translocon co-chaperone

    • Authors : Nurmohamed, Salima Sarah (2021)

  • The transport of proteins into plastids is dependent on how the different translocon components work together. One translocon component, Tic40, possesses functional features found in other proteins such as co-chaperones and may thus work similarly in this capacity. To enhance the understanding of Tic40’s mode of operation, a more basic characterization of the protein itself is required. This study was designed largely to exami...
  • OER000002782.pdf.jpg
  • Journal article

  • Filter paper disks as a matrix for manipulation of recombinant proteins

    • Authors : Ball, Eric H. (2023)

  • AbstractF ilter paper provides an excellent matrix for retention of proteins containing a cellulose binding domain. To use this capability for manipulating recombinant fusion proteins, binding and elution parameters were explored and procedures developed for small scale purification, modification and assay. Proteins were tagged with the cellulose binding domain from the C thermocellum CipB gene via a cleavable linker. Filter paper disks of 6mm&...
  • OER000002419.pdf.jpg
  • Ebooks (Sách điện tử)

  • Homodecameric Rad52 promotes single-position Rad51 nucleation in homologous recombination

    • Authors : Deveryshetty, Jaigeeth (2023)

  • Homologous recombination (HR) is a pathway for the accurate repair of double-stranded DNA breaks. These breaks are resected to yield single-stranded DNA (ssDNA) that are coated by Replication Protein A (RPA). Saccharomyces cerevisiae Rad52 is a mediator protein that promotes HR by facilitating formation of Rad51 nucleoprotein filaments on RPA-coated ssDNA. Canonically, Rad52 has been described to function by displacing RPA to promote Rad51 binding. However, i...
  • OER000002669.pdf.jpg
  • Journal article

  • Nucleic acid quantification with amplicon yield in recombinase polymerase amplification

    • Authors : Roy, Rahul (2023)

  • Amplification-based qPCR provides accurate and sensitive nucleic acid quantification. However, the requirement of temperature cycling and real-time monitoring limits its translation to different settings. Here, we adapted isothermal Recombinase Polymerase Amplification (RPA) reaction to develop a semi-quantitative method that relies on final amplicon yield to estimate initial target nucleic acid copy number. To achieve this, we developed a phenomenological model&#...