Digital resources - Ha Noi university of science and technology

Browsing by Subject tính đặc hiệu

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:

Showing results 1 to 2 of 2

Journal article

An expanded 2-pyridinecarboxaldehyde (2PCA)-based chemoproteomics toolbox for probing protease specificity

Authors : Bridge, Haley N. (2023)

Proteomic profiling of protease-generated N termini, or N terminomics, provides key insights into protease function and specificity. However, current N terminomics technologies have sequence limitations or require specialized synthetic reagents for N-terminal peptide isolation. Here, we introduce an expanded N terminomics toolbox that is based on 2-pyridinecarboxaldehyde (2PCA) reagents. These tools enable efficient enrichment of protein N termini by combining sele...

Journal article

Ketosynthase engineering enhances activity and shifts specificity towards non-native extender units in type I linear polyketide synthase

Authors : Gerlinger, Patrick D. (2023)

Engineering modular type I polyketide synthases (PKS) for the targeted incorporation of non-natural 15 substrates to create variations in the polyketide backbone is a long-standing goal of PKS research. Thus far, 16 most approaches focused on engineering the acyltransferase domain (AT) of PKS, whereas the effects of 17 other ubiquitous domains such as the ketosynthase domain (KS) have received much less attention. In this 18 work, we&#...