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Showing results 1 to 18 of 18
  • OER000002319.pdf.jpg
  • Journal Article

  • A global, integrated view of the ubiquitylation site occupancy and dynamics

    • Authors : Prus, Gabriela (2023)

  • Ubiquitylation regulates virtually all proteins and biological processes in a cell. However, the global site-specific occupancy (stoichiometry) and turnover rate of ubiquitylation have never been quantified. Here, we present the first integrated picture of ubiquitylation site occupancy and half-life. Ubiquitylation occupancy spans four orders of magnitude, but the median ubiquitylation site occupancy is three orders of magnitude lower than that of 20...
  • OER000003037.pdf.jpg
  • Journal article

  • AMPylation is a specific lysosomal protein posttranslational modification in neuronal maturation

    • Authors :  Becker, Tobias (2021)

  • Protein AMPylation is a pervasive posttranslational modification with an emerging role in neurodevelopment. In metazoans the two highly conserved protein AMP-transferases together with a diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques. However, the function of this modification remains largely unknown. Particularly problematic is the localization of thus far identified AMPylated proteins and putative&#x...
  • OER000002664.pdf.jpg
  • Journal article

  • APC couples neuronal mRNAs to multiple kinesins, EB1 and shrinking microtubule ends for bidirectional mRNA motility

    • Authors : Baumann, Sebastian J. (2023)

  • Understanding where in the cytoplasm mRNAs are translated is increasingly recognised as being as important as knowing the timing and level of protein expression. mRNAs are localised via active motor-driven transport along microtubules (MTs) but the underlying essential factors and dynamic interactions are largely unknown. Using biochemical in vitro reconstitutions with purified mammalian proteins, multi-colour TIRF-microscopy (TIRF-M), and interaction kinetics measurements, we s...
  • OER000002501.pdf.jpg
  • Journal article

  • Branched chain amino acid synthesis is coupled to TOR activation early in the cell cycle in yeast

    • Authors : Blank, Heidi M.  (2023)

  • How cells coordinate their metabolism with division determines the rate of cell proliferation. Dynamic patterns of metabolite synthesis during the cell cycle are unexplored. We report the first isotope tracing analysis in synchronous, growing budding yeast cells. Synthesis of leucine, a branched-chain amino acid (BCAA), increased through the G1 phase of the cell cycle, peaking later during DNA replication. Cells lacking Bat1, a mitochondrial&#x...
  • OER000002940.pdf.jpg
  • Journal article

  • Cell-free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs

    • Authors : Do, Hoa Quynh (2021)

  • The Proton-Coupled Folate Transporter (PCFT) is a transmembrane transport protein that controls the absorption of dietary folates in the small intestine. PCFT also mediates uptake of chemotherapeutically used antifolates into tumor cells. PCFT has been identified within lipid rafts observed in phospholipid bilayers of plasma membranes, a micro environment that is altered in tumor cells. The present study aimed at investigating the ...
  • OER000002340.pdf.jpg
  • Journal Article

  • Discovery of novel G-quadruplex stabilizing compounds from medicinal plant & Evaluates their cellular toxicity

    • Authors : Ranjan, Prashant (2023)

  • G-Quadruplexes (G4Q) are higher-order, four-stranded structures that can be formed by repeated guanine tracts in human genomes. In this study, we used a structure-based virtual screening of phytomolecules derived from medicinal plants in order to discover new natural DNA G4Q binders. The top 40 ligands were sorted out based on binding affinity values after completing a docking study on 314 phytomolecule samples with parallel and mixed ...
  • OER000002759.pdf.jpg
  • Journal article

  • Impairments in SHMT2 expression or cellular folate availability reduce oxidative phosphorylation and pyruvate kinase activity

    • Authors : Fiddler, Joanna L. (2023)

  • Serine hydroxymethyltransferase 2 (SHMT2) catalyzes the reversible conversion of 35 tetrahydrofolate (THF) and serine producing THF-conjugated one-carbon units and glycine in 36 the mitochondria. Biallelic SHMT2 variants were identified in humans and suggested to alter the 37 protein’s active site, potentially disrupting enzymatic function. SHMT2 expression has also been 38 shown to decrease with aging in human fibroblasts. Immortalized cells models of total SHMT2 39&#...
  • OER000002590.pdf.jpg
  • Journal article

  • Lipid signatures and inter-cellular heterogeneity of naïve and lipopolysaccharide-stimulated human microglia-like cells

    • Authors : Müller, Max A. (2023)

  • Microglia are non-neuronal cells, which are residing in the central nervous system and are known to play an important role in health and disease. We investigated lipidomic phenotypes of human naïve and stimulated microglia-like cells by atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI). With lateral resolutions between 5 μm and 1.5 μm, we were able to chart lipid compositions of individua...
  • OER000002638.pdf.jpg
  • Journal article

  • RNA-catalyzed RNA Ligation within Prebiotically Plausible Model Protocells

    • Authors : DasGupta, Saurja (2023)

  • Demonstrating RNA catalysis within prebiotically relevant models of primordial cells (protocells) remains a challenge in Origins of life research. Fatty acid vesicles encapsulating genomic and catalytic RNAs (ribozymes) are attractive models for protocells; however, RNA catalysis has largely been incompatible with fatty acid vesicles due to their instability in the presence of Mg2+ at concentrations required for ribozyme function. Here, we report a r...
  • OER000002382.pdf.jpg
  • Journal Article

  • Robust Homeostasis of Cellular Cholesterol via Antithetic Integral Control

    • Authors : Scheepers, Ronél (2023)

  • Although cholesterol is essential for cellular viability and proliferation, it is highly toxic in excess. The concentration of cellular cholesterol must therefore be maintained within tight tolerances, and is thought to be subject to a stringent form of homeostasis known as Robust Perfect Adaptation (RPA). While much is known about the cellular signalling interactions involved in cholesterol regulation, the specific chemical reaction network structur...
  • OER000002974.pdf.jpg
  • Journal article

  • Sphingolipid-Induced Programmed Cell Death Is a Salicylic Acid and EDS1-Dependent Phenotype in Arabidopsis

    • Authors : König, Stefanie (2021)

  • Ceramides and long chain bases (LCBs) are plant sphingolipids involved in the induction of plant programmed cell death (PCD). The fatty acid hydroxylase mutant fah1 fah2 exhibits high ceramide levels and moderately elevated LCB levels. Salicylic acid (SA) is strongly induced in these mutants, but no cell death is visible. To determine the effect of ceramides with different chain lengths, fah1 fah2 was crossed with ceramide ...
  • OER000003050.pdf.jpg
  • Journal article

  • Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

    • Authors : Birke, Ramona (2021)

  • Sulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is validated in live cell imaging experiments in transfected HEK cells and neurons derived from induced pluripotent stem cells (iPSCs). Lastly, we show that&...