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  • OER000003000.pdf.jpg
  • Journal article

  • Transcription initiation at a consensus bacterial promoter proceeds via a “bind-unwind-load-and-lock” mechanism

    • Authors : Mazumder, Abhishek (2021)

  • Transcription initiation starts with unwinding of promoter DNA by RNA polymerase (RNAP) to form a catalytically competent RNAP-promoter complex (RPO). Despite extensive study, the mechanism of promoter unwinding has remained unclear, in part due to the transient nature of intermediates on path to RPo. Here, using single-molecule unwinding-induced fluorescence enhancement to monitor promoter unwinding, and single-molecule fluorescence resonance energy transfer to monitor RNAP...