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Journal article

Ambient temperature crystal structure of Escherichia coli CyaY protein displays alternate conformation

Authors : Shafiei, Alaleh (2023)

Frataxin is a 23 KDa mitochondrial iron-binding protein that is involved in biogenesis of iron sulfur clusters. A deficiency in frataxin leads to Friedreich's ataxia, a progressive neurodegenerative disorder. The bacterial ortholog of eukaryotic mitochondrial frataxin, CyaY, is thought to play a role in iron sulfur cluster assembly as an iron supplier, making it an important target for study. Here, we present the first ambient tem...

Journal Article

Development of in-line anoxic small-angle X-ray scattering and structural characterization of an oxygen-sensing transcriptional regulator

Authors : Illava, Gabrielle (2023)

Oxygen-sensitive metalloenzymes are responsible for many of the most fundamental biochemical processes in nature, from the reduction of di-nitrogen in nitrogenase to the biosynthesis of photosynthetic pigments. However, biophysical characterization of such proteins under anoxic conditions can be challenging, especially at non-cryogenic temperatures. In this study, we introduce the first in-line anoxic small-angle X-ray scattering (anSAXS) system at a major national...

Journal article

Molecular mechanism of quorum sensing inhibition in Streptococcus by the phage protein paratox

Authors : Rutbeek, Nicole R. (2021)

Streptococcus pyogenes, or Group A Streptococcus, is a Gram-positive bacterium that 4 can be both a human commensal and pathogen. Central to this dichotomy are temperate 5 bacteriophages that incorporate into the bacterial genome as a prophage. These genetic 6 elements encode both the phage proteins as well as toxins harmful to the human host. 7 One such conserved phage protein paratox (Prx) is always found enco...

Journal Article

Structural characterization of the Sel1-like repeat protein LceB from Legionella pneumophila

Authors : Penner, Tiffany V. (2023)

Legionella are freshwater Gram-negative bacteria that in their normal environment infect protozoa. However, this adaptation also allows Legionella to infect human alveolar macrophages and cause pneumonia. Central to Legionella pathogenesis are more than 330 secreted effectors, of which there are 9 core effectors that are conserved in all pathogenic species. Despite their importance, the biochemical function of several core effectors remains unclear. ...

Ebooks (Sách điện tử)

X-ray Scattering

Authors : Esther Ares, Alicia (2017)

X-ray scattering techniques are a family of nondestructive analytical techniques. Using these techniques, scientists obtain information about the crystal structure and chemical and physical properties of materials. Nowadays, different techniques are based on observing the scattered intensity of an X-ray beam hitting a sample as a function of incident and scattered angle, polarization, and wavelength. This book is intended to give overviews of the relevant X-ray&#x...