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Showing results 1 to 8 of 8
  • OER000002771.pdf.jpg
  • Journal article

  • A metabolic labeling-based chemoproteomic platform unravels the physiological roles of choline metabolites

    • Authors : Dixit, Aditi (2023)

  • Choline is an essential nutrient for mammalian cells. Our understanding of the cellular functions of choline and its metabolites, independent of their roles as choline lipid metabolism intermediates, remains limited. In addition to fundamental cellular physiology, this knowledge has implications for cancer biology because elevated choline metabolite levels are a hallmark of cancer. Here, we establish the mammalian choline metabolite-interacting proteome by...
  • OER000003009.pdf.jpg
  • Journal article

  • Cell-Tak coating may cause mis-normalization of Seahorse metabolic flux data

    • Authors : Sima, Michal (2021)

  • Metabolic flux investigations of cells and tissue samples are a rapidly advancing tool in diverse research areas. Reliable methods of data normalization are crucial for an adequate interpretation of results and to avoid a misinterpretation of experiments and incorrect conclusions. The most common methods for metabolic flux data normalization are to cell number, DNA and protein. Data normalization may be affected by a variety of factors, such as dens...
  • OER000002360.pdf.jpg
  • Journal Article

  • FruitFire: a luciferase based on a fruit fly metabolic enzyme

    • Authors : Adams Jr., Spencer T. (2023)

  • Firefly luciferase is homologous to fatty acyl-CoA synthetases from insects that are not bioluminescent. Here, we determined the crystal structure of the fruit fly fatty acyl-CoA synthetase CG6178 to 2.5 Å. Based on this structure, we mutated a steric protrusion in the active site to create the artificial luciferase FruitFire, which prefers the synthetic luciferin CycLuc2 to D-luciferin by >1000-fold. FruitFire enabled in vivo bioluminescence...
  • OER000002562.pdf.jpg
  • Journal article

  • Inter-organelle crosstalk supports acetyl-coenzyme A homeostasis and lipogenesis under metabolic stress

    • Authors : Kuna, Ramya S (2023)

  • Proliferating cells rely on acetyl-CoA to support membrane biogenesis and acetylation. Several25 organelle-specific pathways are available for provision of acetyl-CoA as nutrient availability26 fluctuates, so understanding how cells maintain acetyl-CoA flux under such stresses is critically27 important. To this end we applied 13 C isotope tracing cell lines deficient in these mitochondrial28 (ATP-citrate lyase; ACLY-), cytosolic, (acetyl-CoA synthetase (ACSS2-), and...
  • OER000002671.pdf.jpg
  • Journal article

  • Mechanistic insights of glucosome condensate formation by stochastic modeling approaches

    • Authors : Kang, Hye-Won (2023)

  • Human liver-type phosphofructokinase 1 (PFKL) has been shown to play a scaffolder role to recruit and organize glycolytic and gluconeogenic enzymes into a multienzyme metabolic condensate, the glucosome, that regulates glucose flux in living human cells. However, it has been challenging to characterize which factors control phase separation of PFKL and so glucosome condensates in a living cell, thus hampering to understand a mechanism of reversible glucosome&...
  • OER000002955.pdf.jpg
  • Journal article

  • Metabolic response to point mutations reveals principles of modulation of in vivo enzyme activity and phenotype

    • Authors : Bhattacharyya, Sanchari (2021)

  • The relationship between sequence variation and phenotype is poorly understood. Here we use metabolomic analysis to elucidate the molecular mechanism underlying the filamentous phenotype of E. coli strains that carry destabilizing mutations in Dihydrofolate Reductase (DHFR). We find that partial loss of DHFR activity causes reversible filamentation despite SOS response indicative of DNA damage, in contrast to thymineless death (TLD) achieved by complete inhibition of&#...
  • OER000002437.pdf.jpg
  • Ebooks (Sách điện tử)

  • The Genetic Landscape of a Metabolic Interaction

    • Authors : Nguyen, Thuy N. (2023)

  • Enzyme abundance, catalytic activity, and ultimately sequence are all shaped by the need of growing cells to maintain metabolic flux while minimizing accumulation of deleterious intermediates. To quantify how variation in the activity of one enzyme constrains the biochemical parameters and sequence of another, we focused on dihydrofolate reductase (DHFR) and thymidylate synthase (TYMS), a pair of enzymes catalyzing consecutive reactions in folate metabolism. We&#...
  • OER000002377.pdf.jpg
  • Journal Article

  • USP16 is an ISG15 cross-reactive deubiquitinase targeting a subset of metabolic pathway-related proteins

    • Authors : Gan, Jin (2023)

  • The ubiquitin-like modifier ISG15 can modulate host and viral proteins to restrict viral and microbial infections, and act as a cytokine. Its expression and conjugation are strongly upregulated by type I interferons. Here we identify the deubiquitinating enzyme USP16 as an ISG15 cross-reactive protease. Ubiquitin-specific protease 16 (USP16) was found to react with an ISG15 activity-based probe in pull-down experiments using chronic myeloid leu...