Higher Precision in Initial rates may be achievable: A test of a Pseudo-statistical method

Abstract

By graphical means, the Vmax and KM values for galactosidase respectively range between 163 39 and 185 M/min and between 2.07 and 2.77 mg/L; the range by calculations is 177 and 214 M/min and 40 2.45 and 3.311 mg/L, subject to pseudo-statistical remediation. Overall, the ranges of Vmax and KM values 41 for alpha-amylase from both the graphical method and calculation are, respectively, 1.095 to 1.018 42 mM/min and 18.15 to 20.554 g/L.