Structural basis of LRPPRC-SLIRP-dependent translation by the mitoribosome

Abstract

To explore the molecular basis for translation activation in human mitochondria, we used low 90 salt conditions to isolate a mitoribosome:LRPPRC-SLIRP-mRNA complex for cryo-EM. We available under aCC-BY-NC-ND 4.0 International license. was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made bioRxiv preprint doi: https://doi.org/10.1101/2022.06.20.496763; this version posted April 16, 2023. The copyright holder for this preprint (which 4 91 merged particles containing tRNAs in the A- or P-site, as well as an extra density in the vicinity 92 of the mRNA entry channel and applied iterative local-masked refinement and classification with 93 signal subtraction (Extended Data Fig. 1a). It resulted in a 2.9 Å resolution map of the 94 mitoribosome during mRNA delivery to the SSU, with the local resolution for the LRPPRC 95 binding region of ~3.4 Å (Extended Data Fig. 1b,c).