Thông tin tài liệu


Nhan đề : A Zymography technique to study amino acid activation by aminoacyl tRNA synthetases (aaRS): A broad spectrum, high-throughput tool to screen activities of aaRS and their “Urzyme” variants
Tác giả : Patra, Sourav Kumar
Từ khoá : Kỹ thuật; Zymography; axit amin; aminoacyl tRNA synthetase; aaRS; biến thể
Năm xuất bản : 2023
Nhà xuất bản : bioRxiv
Tóm tắt : Amino acyl tRNA synthetases or aaRSs play a key role in assuring the precision of protein 13 translation. They are highly specific for their cognate amino acid and cognate tRNA 14 substrates during protein synthesis, utilizing ATP to ensure that proper assignments are made 15 between amino acid and anticodon. Specific aaRS for each amino acid are present in all cells. 16 We describe a new zymography technique to qualitatively visualize and semi-quantitatively 17 determine the amino acid activation capacity of each type of aaRS molecule by indirect 18 colorimetric detection of released pyrophosphates during the formation of aminoacyl-AMP. 19 Protein samples containing aaRS are subjected to Native PAGE, followed by incubation in 20 buffer containing cognate amino acid and ATP for sufficient time to generate pyrophosphates 21 (PPi) which are then converted to inorganic phosphates by pyrophosphatase treatment.
URI: http://dlib.hust.edu.vn/handle/HUST/23514
Liên kết tài liệu gốc: https://www.biorxiv.org/content/10.1101/2023.02.01.526722v1.full.pdf+html
Trong bộ sưu tập: OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
XEM MÔ TẢ

32

XEM & TẢI

15

Danh sách tệp tin đính kèm:
Ảnh bìa
  • OER000002650.pdf
      Restricted Access
    • Dung lượng : 1,12 MB

    • Định dạng : Adobe PDF



  • Tài liệu được cấp phép theo Bản quyền Creative Commons Creative Commons