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| Title: | Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy |
| Authors: | Bruins, orick |
| Keywords: | phối tử; HaloTag; kính hiển vi; huỳnh quang; đa phương thức; trao đổi |
| Issue Date: | 2023 |
| Publisher: | bioRxiv |
| Abstract: | We introduce exchangeable ligands for fluorescence labeling of HaloTag7 as an alternative to covalently bound probes. The exchangeable ligands open up new possibilities in imaging for a widely used labeling approach, including applications in points accumulation for imaging in nanoscale topography (PAINT), MINFLUX and live-cell, multi-frame stimulated emission depletion (STED) microscopy. We furthermore introduce orthogonal pairs of exchangeable ligands and HaloTags for dual-color PAINT and STED microscopy. |
| URI: | http://dlib.hust.edu.vn/handle/HUST/23549 |
| Link item primary: | https://www.biorxiv.org/content/10.1101/2022.06.20.496706v1.full.pdf+html |
| Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
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