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| Title: | Chemoenzymatic fluorescence labeling of intercellularly contacting cells using lipidated sortase A |
| Authors: | Yamaguchi, Satoshi |
| Keywords: | Ghi nhãn; huỳnh quang; tế bào tiếp xúc; enzyme lipid hóa A |
| Issue Date: | 2023 |
| Publisher: | bioRxiv |
| Description: | Methods to label intercellular contact attract particular attention due to their potential in cell biological and medical applications through analysis of intercellular communications. In this study, a simple and versatile method for chemoenzymatically labeling the intercellularly contacting cell was developed by using a cell-surface anchoring reagent of poly(ethylene glycol)(PEG)-lipid conjugate. The surfaces of each cell in cell pairs of interest were efficiently decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG-lipid, respectively. In the mixture of the two cell populations, the triglycine-modified cells were enzymatically labeled with a fluorescent labeling reagent by contacting with the SrtA-modified cells both on the substrate and in cell suspensions. |
| URI: | http://dlib.hust.edu.vn/handle/HUST/23596 |
| Link item primary: | https://www.biorxiv.org/content/10.1101/2022.05.09.491123v1.full.pdf+html |
| Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
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