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| Trường DC | Giá trị | Ngôn ngữ |
|---|---|---|
| dc.contributor.author | Yamaguchi, Satoshi | - |
| dc.date.accessioned | 2023-11-24T02:17:41Z | - |
| dc.date.available | 2023-11-24T02:17:41Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.other | OER000002732 | vi |
| dc.identifier.uri | http://dlib.hust.edu.vn/handle/HUST/23596 | - |
| dc.description | Methods to label intercellular contact attract particular attention due to their potential in cell biological and medical applications through analysis of intercellular communications. In this study, a simple and versatile method for chemoenzymatically labeling the intercellularly contacting cell was developed by using a cell-surface anchoring reagent of poly(ethylene glycol)(PEG)-lipid conjugate. The surfaces of each cell in cell pairs of interest were efficiently decorated with sortase A (SrtA) and triglycine peptide that were lipidated with PEG-lipid, respectively. In the mixture of the two cell populations, the triglycine-modified cells were enzymatically labeled with a fluorescent labeling reagent by contacting with the SrtA-modified cells both on the substrate and in cell suspensions. | vi |
| dc.description.uri | https://www.biorxiv.org/content/10.1101/2022.05.09.491123v1.full.pdf+html | vi |
| dc.format | vi | |
| dc.language.iso | en | vi |
| dc.publisher | bioRxiv | vi |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Vietnam | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/vn/ | * |
| dc.subject | Ghi nhãn | vi |
| dc.subject | huỳnh quang | vi |
| dc.subject | tế bào tiếp xúc | vi |
| dc.subject | enzyme lipid hóa A | vi |
| dc.subject.lcc | QD505 | vi |
| dc.title | Chemoenzymatic fluorescence labeling of intercellularly contacting cells using lipidated sortase A | vi |
| dc.type | Journal article | vi |
| dc.description.note | CC BY-NC-ND 4.0 | vi |
| Trong bộ sưu tập: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường | |
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