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dc.contributor.authorBirke, Ramona-
dc.date.accessioned2024-01-12T08:21:52Z-
dc.date.available2024-01-12T08:21:52Z-
dc.date.issued2021-
dc.identifier.otherOER000003050vi
dc.identifier.urihttp://dlib.hust.edu.vn/handle/HUST/23915-
dc.description.abstractSulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is validated in live cell imaging experiments in transfected HEK cells and neurons derived from induced pluripotent stem cells (iPSCs). Lastly, we show that Sulfo646 is amenable to STED nanoscopy by recording membranes of SNAP/Halo-surface-labelled human iPSCderived neuronal axons. We therefore provide an avenue for rendering dyes impermeable for exclusive extracellular visualization via self-labelling protein tags.vi
dc.description.urihttps://www.biorxiv.org/content/10.1101/2021.03.16.435698v1.full.pdf+htmlvi
dc.formatPDFvi
dc.language.isoenvi
dc.publisherbioRxivvi
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Vietnam*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/vn/*
dc.subjectfluorophorevi
dc.subjectrhodaminevi
dc.subjectproteinvi
dc.subjectnội soivi
dc.subjectnano STEDvi
dc.subjecttế bàovi
dc.subject.lccTP372.7vi
dc.titleSulfonated rhodamines as impermeable labelling substrates for cell surface protein visualizationvi
dc.typeJournal articlevi
dc.description.noteCC BY 4.0vi
Appears in Collections:OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường

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