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Nhan đề : VC1 catalyzes a key step in the biosynthesis of vicine from GTP in faba bean
Tác giả : Björnsdotter, Emilie
Nadzieja, Marcin
Chang, Wei
Từ khoá : GTP; Convicine biosynthesis; Vicine
Năm xuất bản : 2020
Nhà xuất bản : Biochemical Journal
Tóm tắt : Faba bean is a widely adapted and high-yielding legume cultivated for its protein-rich seeds1. However, the seeds accumulate the anti-nutritional pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia—favism—in the 400 million individuals genetically predisposed by a deficiency in glucose-6-phosphate dehydrogenase2. Here, we identify the first enzyme associated with vicine and convicine biosynthesis, which we name VC1. We show that VC1 co-locates with the major QTL for vicine and convicine content and that the expression of VC1 correlates highly with vicine content across tissues. We also show that low-vicine varieties express a version of VC1 carrying a small, frame-shift insertion, and that overexpression of wild-type VC1 leads to an increase in vicine levels. VC1 encodes a functional GTP cyclohydrolase II, an enzyme normally involved in riboflavin biosynthesis from the purine GTP. Through feeding studies, we demonstrate that GTP is a precursor of vicine both in faba bean and in the distantly related plant bitter gourd. Our results reveal an unexpected biosynthetic origin for vicine and convicine and pave the way for the development of faba bean cultivars that are free from these anti-nutrients, providing a safe and sustainable source of dietary protein.
Mô tả: Tài liệu này được phát hành theo giấy phép CC-BY-NC-ND 4.0
URI: http://dlib.hust.edu.vn/handle/HUST/24615
Liên kết tài liệu gốc: https://www.biorxiv.org/content/10.1101/2020.02.26.966523v1
Trong bộ sưu tập: OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
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