Duyệt theo Kiểu tài liệu "Journal article"
- Ấn phẩm1.7 GHz long-term evolution radiofrequency electromagnetic field with efficient thermal control has no effect on the proliferation of different human cell types(2024) Goh, Jaeseong; Suh, Dongwha; Jeon, SangbongContinuous exposure of various human cell types to 1.7 GHz LTE RF-EMF at specific absorption rate (SAR) of 2 W/Kg for 72 h can induce cellular senescence. To understand the precise cellular effects of LTE RF-EMF, we elaborated the 1.7 GHz RF-EMF cell exposure system used in the previous study by replacing the RF signal generator and developing a software-based feedback system to improve the exposure power stability. This refinement of the 1.7 GHz LTE RF-EMF generator facilitated the automatic regulation of RF-EMF exposure, maintaining target power levels within a 3% range and a constant temperature even during the 72-h exposure period. With the improved experimental setup, we examined the effect of continuous exposure to 1.7 GHz LTE RF-EMF at up to SAR of 8 W/Kg of adipose tissue-derived stem cells and Huh7, HeLa, and B103 cells. Surprisingly, the proliferation of all cell types, which displayed different growth rates, did not change significantly compared with that of the unexposed controls. However, when the thermal control system was turned off and the subsequent temperature increase induced by the RF-EMF was not controlled during continuous exposure to SAR of 8 W/Kg LTE RF-EMF, cellular proliferation increased by 35.2% at the maximum. These observations strongly suggest that the cellular effects attributed to 1.7 GHz LTE RF-EMF exposure were primarily due to the induced thermal changes, rather than the RF-EMF exposure itself.
- Ấn phẩm16-channel SiPM high-frequency readout with time-over-threshold discrimination for ultrafast time-of-flight applications(Springer Nature, 2023) Vanessa Nadig,... [et. al.]Giới thiệu hệ thống đọc 16 kênh với phương pháp phân biệt ngưỡng theo thời gian (ToT), cho phép xử lý tín hiệu nhanh và chính xác hơn. Kết quả cho thấy hiệu năng định thời được nâng cao rõ rệt, mở ra tiềm năng ứng dụng trong chẩn đoán hình ảnh y học hạt nhân và các hệ thống yêu cầu độ phân giải thời gian cực cao.
- Ấn phẩm2D transition metal carbides (MXenes) in metal and ceramic matrix composites(Springer Nature, 2021) Brian C. Wyatt; Srinivasa Kartik Nemani; Babak Anasori.Tổng quan về cacbua kim loại chuyển tiếp hai chiều (MXenes) – một nhóm vật liệu nano gồm cacbua, nitride và carbonitride của kim loại chuyển tiếp. MXenes được ứng dụng như chất gia cường và độn tiên tiến trong composite nền kim loại và gốm, giúp nâng cao độ dẫn điện, độ bền cơ học, tính chịu nhiệt và khả năng chống ăn mòn. Tác giả phân tích mối tương tác giữa MXenes và nền vật liệu, ảnh hưởng của cấu trúc lớp, phương pháp tổng hợp và xử lý bề mặt. Bài báo cũng chỉ ra tiềm năng ứng dụng của MXenes trong lưu trữ năng lượng, xúc tác, chống oxy hóa và chế tạo vật liệu siêu nhẹ cho công nghiệp hiện đại.
- Ấn phẩm30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC(bioRxiv, 2023) Srinivas, PoojaAcquired ribosomal RNA (rRNA) methylation has emerged as a significant mechanism of aminoglycoside resistance in pathogenic bacterial infections. Modification of a single nucleotide in the ribosome decoding center by the aminoglycoside-resistance 16S rRNA (m7G1405) methyltransferases effectively blocks the action of all 4,6-deoxystreptamine ring-containing aminoglycosides, including the latest generation of drugs. To define the molecular basis of 30S subunit recognition and G1405 modification by these enzymes, we used a S-adenosyl-L-methionine (SAM) analog to trap the complex in a post-catalytic state to enable determination of an overall 3.0 Å cryo-electron microscopy structure of the m7G1405 methyltransferase RmtC bound to the mature Escherichia coli 30S ribosomal subunit. This structure, together with functional analyses of RmtC variants, identifies the RmtC N-terminal domain as critical for recognition and docking of the enzyme on a conserved 16S rRNA tertiary surface adjacent to G1405 in 16S rRNA helix 44 (h44). To access the G1405 N7 position for modification, a collection of residues across one surface of RmtC, including a loop that undergoes a disorder to order transition upon 30S subunit binding, induces significant distortion of h44.
- Ấn phẩm5’ modifications to CRISPR Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage(Biochemical Journal, 2020) Mullally, Grace; Aelst, Kara van; Naqvi, Mohsin MA key aim in exploiting CRISPR-Cas is the engineering of gRNA to introduce additional functionalities, ranging from small nucleotide changes that increase efficiency of on-target binding to the inclusion of large functional RNA aptamers and ribonucleoproteins (RNPs. Interactions between gRNA and Cas9 are crucial for RNP complex assembly but several distinct regions of the gRNA are amenable to modification. Using a library of modified gRNAs, we used in vitro ensemble and single-molecule assays to assess the impact of RNA structural alterations on RNP complex formation, R-loop dynamics, and endonuclease activity. Our results indicate that R-loop formation and DNA cleavage activity are essentially unaffected by gRNA modifications of the Upper Stem, first Hairpin and 3’ end. In contrast, 5’ additions of only two or three nucleotides reduced R-loop formation and cleavage activity of the RuvC domain relative to a single nucleotide addition. Such gRNA modifications are a common by-product of in vitro transcribed gRNA. We also observed that addition of a 20 nt RNA hairpin to the 5’ end supported formation of a stable ~9 bp R-loop that could not activate DNA cleavage. These observations will assist in successful gRNA design.
- Ấn phẩmA 2050 perspective on the role for carbon capture and storage in the European power system and industry sector(Elsevier B.V., 2021) Franziska, HolzCarbon Capture and Storage (CCS) might be a central technology to reach the decarbonisation goals of the European energy system. However, CCS deployment faces multiple economic, technological, and infrastructure challenges. Related literature tends to only focus on certain aspects of the CCS technology or to be limited to a particular sector perspective. In contrast, this paper presents a holistic modelling framework to analyse the longterm perspectives of CCS in Europe by extending the typical analysis from the electricity sector to the industry sector, and by including the CO2 infrastructure level with CO2 pipelines and storage. To this end, we use state-ofthe- art models of the electricity sector (generation investment and electricity grid models), the industry sector, as well as the CO2 infrastructure sector. This unique modelling framework analyses the feasibility and costs of CCS deployment in the European Union towards 2050 in three scenarios with the same ambitious climate policy target (~85% CO2 emissions reduction). The main insights on the deployment of CCS in Europe hinges on two factors: i) the development of low-cost power generation technologies with carbon capture (coal and/or gasfired), and ii) a sufficiently high CO2 price to compensate for the costs of deploying the CO2 transport infrastructure. Once CO2 transport infrastructure is available, CCS will be a preferred mitigation option for the industry sector emissions. The joint use of CO2 infrastructure by the electricity and the industry sector allows for economies of scale and economies of density. In the long term, CCS cannot achieve the 100% decarbonisation target of the energy sector because the technology can only capture 80–90% of the CO2 emissions of thermal power plants. Moreover, the advantages of CCS in terms of energy system costs compared to a system without CCS is rather small, in the range of 2%. It crucially depends on the costs of renewables and the costs of their integration in the electricity grid.
- Ấn phẩmA benchmark of protein solubility prediction methods on UDP-dependent glycosyltransferases(Biochemical Journal, 2020) Ghomi, Fatemeh Ashari; Kittilä, Tiia; Welner, Ditte HededamUDP-dependent glycosyltransferases (UGTs) are enzymes that glycosylate a wide variety of natural products, thereby modifying their physico-chemical properties, i.e. solubility, stability, reactivity, and function. To successfully leverage the UGTs in biocatalytic processes, we need to be able to screen and characterise them in vitro, which requires efficient heterologous expression in amenable hosts, preferably Escherichia coli. However, many UGTs are insoluble when expressed in standard and attempted optimised E. coli conditions, resulting in many unproductive and costly experiments. To overcome this limitation, we have investigated the performance of 11 existing solubility predictors on a dataset of 57 UGTs expressed in E. coli. We show that SoluProt outperforms other methods in terms of both threshold-independent and threshold-dependent measures. Among the benchmarked methods, only SoluProt is significantly better than random predictors using both measures. Moreover, we show that SoluProt uses a threshold for separating soluble and insoluble proteins that is optimal for our dataset. Hence, we conclude that using SoluProt to select UGT sequences for in vitro investigation will significantly increase the success rate of soluble expression, thereby minimising cost and enabling efficient characterisation efforts for biocatalysis research
- Ấn phẩmA binary arginine methylation switch on histone H3 Arginine 2 regulates its interaction with WDR5(Biochemical Journal, 2020) Lorton, Benjamin M; Harijan, Rajesh K; Burgos, Emmanuel SUsing highly accurate quantitative binding analysis combined with high-resolution crystal structures of WDR5 in complex with unmodified (me0) and me1/me2s L-Arginine amino acids and in complex with H3R2me1 peptide, we provide a rigorous biochemical study of this important biological interaction. Despite modest structural differences at the binding interface, our study supports an interaction model regulated by a binary arginine methylation switch: H3R2me2a prevents interaction with WDR5, whereas H3R2me0/me1/me2s are equally permissive.
- Ấn phẩmA biophysical framework for double-drugging kinases(bioRxiv, 2023) Kim, C.Orthosteric inhibition of kinases has been challenging due to the conserved active site architecture of kinases and emergence of resistance mutants. Simultaneous inhibition of distant orthosteric and allosteric sites, which we refer to as “double-drugging”, has recently been shown to be effective in overcoming drug resistance. However, detailed biophysical characterization of the cooperative nature between orthosteric and allosteric modulators has not been undertaken. Here, we provide a quantitative framework for double-drugging of kinases employing isothermal titration calorimetry, Förster resonance energy transfer, coupled-enzyme assays, and X-ray crystallography. We discern positive and negative cooperativity for Aurora A kinase (AurA) and Abelson kinase (Abl) with different combinations of orthosteric and allosteric modulators. We find that a conformational equilibrium shift is the main principle governing this cooperative effect.
- Ấn phẩmA biosensor to gauge protein homeostasis resilience differences in the nucleus compared to cytosol of mammalian cells(bioRxiv, 2021) Raeburn, Candice B.An extensive network of chaperones and other proteins maintain protein homeostasis and guard against inappropriate protein aggregation that is a hallmark of neurodegenerative diseases. Using a fluorescence resonance energy-based biosensor that simultaneously reports on intact cellular chaperone holdase activity and detrimental aggregation propensity, we investigated the buffering capacity of the systems managing protein homeostasis in the nucleus of the human cell line HEK293 compared to the cytosol. We found that the nucleus showed lower net holdase activity and reduced capacity to suppress protein aggregation, suggesting that the nuclear quality control resources are less effective compared to those in the cytosol. Aggregation of mutant huntingtin exon 1 protein (Httex1) in the cytosol appeared to deplete cytosolic chaperone supply by depleting holdase activity.
- Ấn phẩmA body detection inversion effect revealed by a large-scale inattentional blindness experiment(BioRxiv, 2025) Marco Gandolfo; Marius V. PeelenNghiên cứu tác động của hiện tượng mù chú ý (inattentional blindness) đến khả năng phát hiện hình dáng cơ thể người. Với dữ liệu từ hơn 13.000 người tham gia, kết quả cho thấy cơ thể người ở tư thế thẳng đứng được phát hiện nhanh và chính xác hơn so với hình dạng bị đảo ngược hoặc hình dáng thực vật, bất kể sự tương đồng về đặc điểm thị giác. Phát hiện này chứng minh rằng hệ thống thị giác con người đã thích ứng để phát hiện nhanh sự hiện diện của đồng loại. Nghiên cứu cũng ghi nhận sự khác biệt đáng kể về khả năng phát hiện theo giới tính và độ tuổi.
- Ấn phẩmA common algorithm for confidence judgements across visual, auditory and audio-visual decisions(BioRxiv, 2025)Nghiên cứu này phân tích cơ chế tính toán của sự tự tin trong các quyết định cảm giác. Sử dụng mô hình tính toán, các tác giả so sánh ba lớp mô hình phổ biến (cường độ bằng chứng không tỉ lệ, cường độ bằng chứng có tỉ lệ và mô hình Bayes) trong các nhiệm vụ nhận thức thị giác, thính giác và đa giác quan (thị - thính). Kết quả cho thấy mô hình cường độ bằng chứng có tỉ lệ vượt trội hơn hẳn, có khả năng dự đoán hành vi trong các nhiệm vụ tích hợp đa giác quan. Người tham gia không chỉ dựa vào giác quan mạnh nhất, mà tích hợp đồng thời thông tin thị giác và thính giác, sử dụng một thước đo bất định cảm giác độc lập với phương thức. Kết quả cung cấp bằng chứng về một thuật toán chung chi phối sự tự tin trong các quyết định cảm giác, khẳng định tính ứng dụng rộng của mô hình này.
- Ấn phẩmA common polymorphism that protects from cardiovascular disease increases fibronectin processing and secretion(bioRxiv, 2021) Soubeyrand, SébastienFibronectin (FN1) is an essential regulator of homodynamic processes and tissue remodeling which has been proposed to contribute to atherosclerosis. Moreover, recent large scale genome wide association studies have linked common genetic variants within the FN1 gene to coronary artery disease (CAD) risk. Genome-wide Association Studies (GWAS) have identified hundreds of common single nucleotide polymorphisms (SNPs) that associate with cardiovascular disease (CAD) risk 1–3 Although GWAS signals are enriched for expression quantitative trait loci (eQTLs) indicating measurable impacts on transcription, the identification of causal genes is challenging since 1) the vast majority of common trait related SNPs do not overlap protein coding genes and 2) are eQTLs for multiple genes 4,5 62 .
- Ấn phẩmA Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients(Biochemical Journal, 2019) Jensen, Dorte B. Bekker; Val, Ana Martínez del; Steigerwald, SophiaState-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per second with attomolar sensitivity. Here we describe a compact and robust quadrupole-orbitrap mass spectrometer equipped with a front-end High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Interface. The performance of the Orbitrap Exploris 480 mass spectrometer is evaluated in data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes in combination with FAIMS. We demonstrate that different compensation voltages (CVs) for FAIMS are optimal for DDA and DIA, respectively. Combining DIA with FAIMS using single CVs, the instrument surpasses 2500 unique peptides identified per minute. This enables quantification of >5000 proteins with short online LC gradients delivered by the Evosep One LC system allowing acquisition of 60 samples per day. The raw sensitivity of the instrument is evaluated by analyzing 5 ng of a HeLa digest from which >1000 proteins were reproducibly identified with 5 minute LC gradients using DIA-FAIMS. To demonstrate the versatility of the instrument we recorded an organ-wide map of proteome expression across 12 rat tissues quantified by tandem mass tags and label-free quantification using DIA with FAIMS to a depth of >10,000 proteins.
- Ấn phẩmA comparison of DC- versus AC-based minigrids for cost-effective electrification of rural developing communities(Elsevier Ltd, 2019) Nicholas, Nixon OpiyoSub-Saharan Africa continues to suffer energy poverty due to low grid expansion rates necessitated by low economic activities in those regions, sparse population distribution coupled with low household load demands, and insufficient power generation. On the other hand, small solar power microgeneration systems have emerged as potential alternatives to grid electrifications, enabling households to make modest investments into their power systems, and to modify those systems according to their changing economic and power demand circumstances. For rural social-economic development, electricity-beyond-lighting is needed. Without the grid, the only alternative is minigrids based on locally available renewable energy resources. In this work, we compare the merits and demerits of DC and AC coupled systems as pertains to costs, efficiencies, and overall performances. Research shows that power conversion stages are the biggest points of power losses in minigrids and therefore avoiding many conversion stages lead to improved overall system efficiencies. Research also shows that the best performances are realized when DC-inherent appliances are supplied with power from DC-coupled networks, supplied by distributed DC power generators such as PV. Simulation results show that when given choices, consumers choose to connect to DC networks with decentralized storage due to lowest operating costs, ease of expansion, and overall better performances when compared to other networks.
- Ấn phẩmA Comparison of Quantitative Mass Spectrometric Methods for Drug Target Identification by Thermal Proteome Profiling(bioRxiv, 2023) George, Amy L.Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target and off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due to target engagement requires high quantitative accuracy and consistent detection. Isobaric tandem mass tags (TMT) are used to multiplex samples and increase quantification precision in TPP analysis by data-dependent acquisition (DDA). However, advances in data-independent acquisition (DIA) can provide higher sensitivity and protein coverage with reduced costs and sample preparation steps. Herein, we explored the performance of different DIA-based label-free quantification (LFQ) approaches compared to TMT-DDA for thermal shift quantitation. Acute myeloid leukaemia (AML) cells were treated with losmapimod, a known inhibitor of MAPK14 (p38α).
- Ấn phẩmA Comparison of Sleep-Based and Retrieval-Mediated Memory Consolidation Using Sigma-Band Activity(BioRxiv, 2025) Hayley B. Caldwell; Alex Chatburn.; Kurt LushingtonBài báo so sánh hai cơ chế củng cố trí nhớ: dựa trên giấc ngủ và dựa trên thực hành gọi lại (retrieval-mediated). Dữ liệu EEG sigma-band (11–16 Hz) được sử dụng làm chỉ báo sóng thoi giấc ngủ. Kết quả không xác nhận giả thuyết rằng sigma-band activity tăng cường trí nhớ của các mục được mã hóa yếu; ngược lại, có tác động tiêu cực. Những mục được mã hóa mạnh vẫn được củng cố tốt, và thực hành gọi lại và ôn tập mang lại hiệu quả nhớ tốt hơn so với ngủ hoặc nghỉ. Nghiên cứu đề xuất phương pháp luận để so sánh các hình thức củng cố trí nhớ trong các paradigm khác nhau.
- Ấn phẩmA complex IRES at the 5’-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate(Biochemical Journal, 2019) Neupane, Ritam; Pisareva, Vera P; Rodríguez, Carlos FRNA viruses are pervasive entities in the biosphere with significant impact in human health and economically important livestock. As strict cellular parasites, RNA viruses abuse host resources, redirecting them towards viral replication needs. Taking control of the cellular apparatus for protein production is a requirement for virus progression and diverse strategies of cellular mimicry and/or ribosome hijacking evolved to ensure this control. Especially in complex eukaryotes, translation is a sophisticated process, with multiple mechanisms acting on ribosomes and mRNAs. The initiation stage of translation is specially regulated, involving multiple steps and the engagement of numerous initiation factors some of them of high complexity. The use of structured RNA sequences, called Internal Ribosomal Entry Sites (IRES), in viral RNAs is a widespread strategy for the exploitation of eukaryotic initiation. Using a combination of electron cryo-microscopy (cryo-EM) and reconstituted translation initiation assays with native components, we characterized how a novel IRES at the 5’-UTR of a viral RNA assembles a functional translation initiation complex via an uAUG intermediate, redirecting the cellular machinery for protein production towards viral messengers. The IRES features a novel extended, multi-domain architecture, circling the 40S head, leveraging ribosomal sites not previously described to be exploited by any IRES. The structures and accompanying functional data, illustrate the importance of 5’-UTR regions in translation regulation and underline the relevance of the untapped diversity of viral IRESs. Given the large number of new viruses metagenomic studies have uncovered, the quantity and diversity of mechanisms for translation hijacking encrypted in viral sequences may be seriously underestimated. Exploring this diversity could reveal novel avenues in the fight against these molecular pathogens.
- Ấn phẩmA composite filter for low FDR of protein-protein interactions detected by in vivo cross-linking(Biochemical Journal, 2020) Jong, Luitzen de; Roseboom, Winfried; Kramer, GertjanIn vivo chemical cross-linking combined with LCMSMS of digested extracts (in vivo CX-MS) can reveal stable and dynamic protein-protein interactions at a proteome wide-scale and at the peptide level. In vivo CX-MS requires a membrane permeable and cleavable cross-linker that enables isolation of target peptides and a fast and sensitive search engine to identify the linked peptides. Here we explore the use of the search engine pLink 2 for analysis of a previously obtained LCMSMS dataset from exponentially growing Bacillus subtilis treated in culture with the cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (BAMG). Cross-linked peptide pairs were identified by pLink 2 in very short time at an overall FDR of < 5%. To also obtain a FDR < 5% for inter-protein cross-linked peptide pairs additional thresholds values were applied for matched fragment intensity and for the numbers of unambiguous y and b ions to be assigned for both composite peptides. Threshold values were based on a set of decoy sequences from yeast and human sequence databases. Also the mass- and charge-dependent retention times of target peptides purified by diagonal strong cation exchange chromatography were used as a criterion to distinguish true from false positives. After this filtering, pLink 2 identified more than 80% of previously reported protein-protein interactions. In addition the use of pLink 2 revealed interesting new inter-protein cross-linked peptide pairs, among others showing interactions between the global transcriptional repressor AbrB and elongation factor Tu and between the essential protein YlaN of unknown function and the ferric uptake repressor Fur.
- Ấn phẩmA comprehensive landscape of 60S ribosome biogenesis factors(bioRxiv, 2023) Sailer, CarolinEukaryotic ribosome biogenesis is facilitated and regulated by numerous ribosome biogenesis factors (RBFs). High-resolution cryo-EM maps have defined the molecular interactions of RBFs during maturation, but many transient and dynamic interactions, particularly during early assembly, remain uncharacterized. Using quantitative proteomics and crosslinking coupled to mass spectrometry (XL-MS) data from a extensive set of pre-ribosomal particles, we derived a comprehensive and timeresolved interaction map of RBF engagement during 60S maturation. A novel filter that efficiently eliminates false positive interactions and integration of our MS data with known structural information allowed us to localize 22 unmapped RBFs to specific biogenesis intermediates and to identify 9 proteins that represent potentially new RBFs.
- Ấn phẩmA Comprehensive Review of Solid-phase Additive Techniques: Insights into Friction Stir Additive Manufacturing (FSAM) and Additive Friction Stir Deposition (AFSD)(Elsevier B.V., 2025) K. N. Nouranga; B. N. Prashanth; T. Ram Prabhu.Tổng quan các kỹ thuật đắp dần trạng thái rắn, tập trung vào Friction Stir Additive Manufacturing (FSAM) và Additive Friction Stir Deposition (AFSD). Nội dung phân tích nguyên lý, thông số quy trình, tiến triển cấu trúc vi mô, tính chất cơ học và các ứng dụng tiềm năng trong công nghiệp của các kỹ thuật này.
- Ấn phẩmA comprehensive review on smart manufacturing using machine learning applicable to fused deposition modeling(Elsevier B.V., 2025) Swapnil Deokar; Narendra Kumar; Ravi Pratap Singh.Các ứng dụng của học máy trong sản xuất thông minh, đặc biệt cho FDM. ML được sử dụng để dự đoán độ nhám bề mặt, giám sát chất lượng dự đoán và tối ưu hóa hiệu suất sản xuất. Nghiên cứu cũng phân tích thách thức và tiềm năng của việc tích hợp ML để nâng cao hiệu quả và chất lượng sản phẩm trong FDM.
- Ấn phẩmA comprehensive review on thermal management of electronic devices(Springer Nature, 2025) Amol R. Dhumal; Atul P. Kulkarni; Nitin H. Ambhore.Tổng quan về quản lý nhiệt trong thiết bị điện tử, đánh giá các phương pháp làm mát hiện nay nhằm duy trì hiệu suất ổn định và độ bền của thiết bị. Các kỹ thuật được xem xét gồm: dẫn nhiệt, đối lưu, làm mát bằng chất lỏng, vật liệu dẫn nhiệt cao, và các giải pháp tích hợp. Bài viết cũng nêu các thách thức hiện tại và xu hướng nghiên cứu TM cho thiết bị điện tử tiên tiến.
- Ấn phẩmA computational approach to sequential decision optimization in energy storage and trading(EconStor, 2021) Paolo, FalboDue to recent technical progress, battery energy storages are becoming a viable option in the power sector. Their optimal operational management focuses on load shift and shaving of price spikes. However, this requires optimally responding to electricity demand, intermittent generation, and volatile electricity prices. More importantly, such optimization must take into account the so-called deep discharge costs, which have a significant impact on battery lifespan. We present a solution to a class of stochastic optimal control problems associated with these applications. Our numerical techniques are based on efficient algorithms which deliver a guaranteed accuracy.