Journal ArticleAuthors : Elena-Real, Carlos A. (2023)
NMR studies of large biomolecular machines and highly repetitive proteins remain
challenging due to the difficulty of assigning signals to individual nuclei. Here, we present an efficient
strategy to address this challenge by engineering a Pyrococcus horikoshii tRNA/alanyl-tRNA
synthetase pair that enables the incorporation of up to three isotopically labeled alanine residues in a
site-specific manner using in vitro protein expression. We have d...