Browsing by Author Zemella, Anne

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  • OER000000829.pdf.jpg
  • Journal article

  • Authors : Dhandapani, Priyavathi; Dondapati, Srujan Kumar; Zemella, Anne (-)

  • Calcium imaging is an important functional tool for addressing ion channels, transporters and pumps for drug screening in living cells. Depicted eukaryotic cell-free systems utilize microsomes, derived from endoplasmic reticulum to incorporate the synthesized membrane proteins. Absence or inadequate amount of carboxylesterase in the endoplasmic reticulum of eukaryotic cells, which is necessary to cleave the acetoxymethyl ester moiety of the chemical calcium indicators, advo...