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Browsing by Author Zacchi, Lucia F

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Showing results 2 to 4 of 4
  • OER000000926.pdf.jpg
  • Journal article

  • Glycoproteomic measurement of site-specific polysialylation

    • Authors : Pelingon, Ruby; Pegg, Cassandra L; Zacchi, Lucia F (2020)

  • Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans. Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer. Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics. PSA chains vary in length, composition, and linkages, while the specif...
  • OER000000830.pdf.jpg
  • Journal article

  • Identification of novel glycosylation events on human serum-derived Factor IX

    • Authors : Pegg, Cassandra L; Zacchi, Lucia F; Recinos, Dinora Roche (2020)

  • Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade. Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates. Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and&...
  • OER000000806.pdf.jpg
  • Journal article

  • S-Trap eliminates cell culture media polymeric surfactants for effective proteomic analysis of mammalian cell bioreactor supernatants

    • Authors : Zacchi, Lucia F; Recinos, Dinora Roche; Otte, Ellen (2020)

  • Proteomic analysis of bioreactor supernatants can inform on cellular metabolic status, viability, and productivity, as well as product quality, which can in turn help optimize bioreactor operation. Incubating mammalian cells in bioreactors requires the addition of polymeric surfactants such as Pluronic F68, which reduce the sheer stress caused by agitation. However, these surfactants are incompatible with mass spectrometry proteomics and must be eliminated during sampl...