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  • Authors : Müller, Marie-Caroline (2023)

  • Glutamine synthetases catalyze the ATP-dependent ammonium assimilation, the initial step of nitrogen 18 acquisition that must be tightly regulated to fit cellular needs. While their catalytic mechanisms and 19 regulation are well-characterized in bacteria and eukaryotes, only limited knowledge exists about the 20 archaeal representatives. Here, we natively purified the glutamine synthetases type I-α from 21 Methanothermococcus thermolithotrophicus and Methermicoccus shengliensis, two...