Digital resources - Ha Noi university of science and technology

Browsing by Subject E. coli RNA polymerase-λPR

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:

Showing results 1 to 1 of 1

Journal article

Kinetic-mechanistic evidence for which E. coli RNA polymerase-λPR open promoter complex initiates and for stepwise disruption of contacts in bubble collapse

Authors : Plaskon, Dylan; Henderson, Kate; Felth, Lindsey (2020)

To report the kinetics of initial transcription at the λPR promoter at 25 °C and 37 °C. Rapid-quench mixing is used to determine overall rates of full-length RNA synthesis and rate constants for individual nucleotide addition steps at two nucleotide conditions (designated high UTP, low UTP) for comparison with the 19 °C results. These studies address the questions of which OC conformation is capable of binding nucleotides and which specific...