Browsing by Subject IG CRISPR-Cas

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  • Authors : Dhingra, Yukti (2023)

  • CRISPR-Cas adaptive immune systems uptake short ‘spacer’ sequences from foreign DNA and 8 incorporate them into the host genome to serve as templates for crRNAs that guide interference 9 against future infections. Adaptation in CRISPR systems is mediated by Cas1-Cas2 complexes 10 that catalyze integration of prespacer substrates into the CRISPR array. Many DNA targeting 11 systems also require Cas4 endonucleases for functional spacer acquisition. Cas4 selects...