Browsing by Subject UDP-dependent glycosyltransferase

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  • OER000000845.pdf.jpg
  • Journal article

  • Authors : Ghomi, Fatemeh Ashari; Kittilä, Tiia; Welner, Ditte Hededam (2020)

  • UDP-dependent glycosyltransferases (UGTs) are enzymes that glycosylate a wide variety of natural products, thereby modifying their physico-chemical properties, i.e. solubility, stability, reactivity, and function. To successfully leverage the UGTs in biocatalytic processes, we need to be able to screen and characterise them in vitro, which requires efficient heterologous expression in amenable hosts, preferably Escherichia coli. However, many UGTs are insoluble when expressed in...