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Journal article

A Zymography technique to study amino acid activation by aminoacyl tRNA synthetases (aaRS): A broad spectrum, high-throughput tool to screen activities of aaRS and their “Urzyme” variants

Authors : Patra, Sourav Kumar (2023)

Amino acyl tRNA synthetases or aaRSs play a key role in assuring the precision of protein 13 translation. They are highly specific for their cognate amino acid and cognate tRNA 14 substrates during protein synthesis, utilizing ATP to ensure that proper assignments are made 15 between amino acid and anticodon. Specific aaRS for each amino acid are present in all cells. 16 We describe a new zymography technique to qualitat...