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  • Nghiên cứu chế tạo que thử phát hiện nhanh độc tố bền nhiệt shiga type 2 của Escherichia coli

    • Authors : Tô Lan Anh;  Advisor : Nguyễn Văn Hoàng; Lê Quang Hòa (2018)

  • Tổng quan vi khuẩn E.coli sinh độc tố shiga, độc tố shiga, các phương pháp phát hiện độc tố shiga type 2. Nêu phương pháp nghiên cứu. Kết quả lựa chọn kháng thể phù hợp cho quá trình chế tạo que thử, tạo dung dịch hạt vàng nano, cộng hợp hạt vàng với kỹ thuật phát hiện và cố định trên màng cộng hợp, cố định kháng thể lên màng nitrocellulose.
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  • Journal article

  • Novel glycoside hydrolase family enzymes from Escherichia coli are associated with osmo-regulated periplasmic glucan synthesis

    • Authors : Motouchi, Sei (2023)

  • Most Gram-negative bacteria synthesize osmo-regulated periplasmic glucans (OPG) in the periplasm or extracellular space. Many pathogens lose their pathogenicity by knocking out opgG, an OPG-related gene indispensable for OPG synthesis. However, the biochemical functions of OpgG and OpgD, a paralog of OpgG, have not been elucidated. In this report, structural and functional analyses of OpgG and OpgD from Escherichia coli revealed that these proteins are β-1,2-gluca...
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  • Journal article

  • Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation

    • Authors : Kolata, Piotr (2021)

  • Respiratory complex I is a multi-subunit membrane protein complex that reversibly couples NADH oxidation and ubiquinone reduction with proton translocation against trans-membrane potential. Complex I from Escherichia coli is among the best functionally characterizedcomplexes, but its structure remains unknown, hindering further mechanistic studies to understand the enzyme coupling mechanism. Here we describe the single particle cryo electron microscopy (cryo-EM) structure of th...
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  • Journal article

  • The role of glutathione in periplasmic redox homeostasis and oxidative protein folding in Escherichia coli

    • Authors : Knoke, Lisa R. (2023)

  • The thiol redox balance in the periplasm of E. coli depends on the DsbA/B pair for oxidative 14 power and the DsbC/D system as its complement for isomerization of non-native disulfides. 15 While the standard redox potentials of those systems are known, the in vivo redox potential 16 imposed onto protein thiol disulfide pairs in the periplasm remains unknown. Here, we used 17 genetically encoded redox probes (roGFP2 ...
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  • Journal article

  • The role of glutathione in periplasmic redox homeostasis and oxidative protein folding in Escherichia coli

    • Authors : Knoke, Lisa R. (2023)

  • The thiol redox balance in the periplasm of E. coli depends on the DsbA/B pair for oxidative 14 power and the DsbC/D system as its complement for isomerization of non-native disulfides. 15 While the standard redox potentials of those systems are known, the in vivo redox potential 16 imposed onto protein thiol disulfide pairs in the periplasm remains unknown. Here, we used 17 genetically encoded redox probes (roGFP2 ...
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  • Journal article

  • XACT-seq comprehensively defines the promoter-position and promoter-sequence determinants for initial-transcription pausing

    • Authors : Winkelman, Jared T; Pukhrambam, Chirangini; Vvedenskaya, Irina O (2019)

  • Pausing by RNA polymerase (RNAP) during transcription elongation, in which a translocating RNAP uses a “stepping” mechanism, has been studied extensively, but pausing by RNAP during initial transcription, in which a promoter-anchored RNAP uses a “scrunching” mechanism, has not. We report a method that directly defines RNAP-active-center position relative to DNA in vivo with single-nucleotide resolution (XACT-seq; crosslink-between-active-center-and-template sequencing). We apply this...