Journal articleAuthors : Carman, Peter J. (2021)
Biochemical studies require large protein quantities, which are typically obtained using bacterial
expression. However, the folding machinery of bacteria is inadequate for many mammalian
proteins, which additionally undergo posttranslational modifications (PTMs) that bacteria, yeast,
or insect cells cannot perform. Many proteins also require native N- and C-termini and cannot
tolerate extra tag amino acids for function. Tropomyosin, a coiled coil ...