- Journal article
Authors : Ball, Eric H. (2023) - AbstractF
ilter paper provides an excellent matrix for retention of proteins containing a cellulose binding
domain. To use this capability for manipulating recombinant fusion proteins, binding and elution
parameters were explored and procedures developed for small scale purification, modification and
assay. Proteins were tagged with the cellulose binding domain from the C thermocellum CipB gene via
a cleavable linker. Filter paper disks of 6mm&...
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- Journal Article
Authors : Cordoba, John J (2023) - In eukaryotes, DNA polymerase α-primase (pol-prim) initiates DNA synthesis during replication, generating the first ~30 nucleotides of nascent strands.1–5 Pol-prim is unique among replicative polymerases in its ability to perform de novo synthesis from a single-stranded DNA template; the primers it creates are required for further synthesis by the processive polymerases ε and δ that perform the bulk of nascent strand synthesis6,7. Pol-prim plays a particularly...
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- Journal article
Authors : Frei, Michelle S. (2021) - HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased
fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9
with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single
fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased
brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a...
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- Journal article
Authors : witucki (2023) - The loss of cystathionine β-synthase (CBS), an important homocysteine (Hcy)-metabolizing enzyme or
the loss of PHF8, an important histone demethylase participating in epigenetic regulation, causes severe
mental retardation in humans. Similar neuropathies were also observed in Cbs-/- and Phf8-/- mice. How
CBS or PHF8 depletion can cause neuropathy was unknown. To answer this question, we examined a
possible interaction between PHF8 and CBS u...
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- Journal article
Authors : Barik, Sushanta Kumar (2021) - Acquired immunodeficiency syndrome is one of the most
important diseases caused by human immunodeficiency virus. Understanding its molecular
pathogenesis is essential to manage the disease at the population level. In this study, a quantitative analysis of plasma proteins was carried out in drug resistant and drug respondent patients using the SWATH-MS. Sequential window acquisition of all theoretical mass spectra (SWATH-MS) is a prime technique to seek...
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- Journal Article
Authors : Enustun, Eray (2023) - In the arms race between bacteria and bacteriophages (phages), some large-genome jumbo
phages have evolved a protein shell that encloses their replicating genome to protect it
against DNA-targeting immune factors. By segregating the genome from the host cytoplasm,
however, the “phage nucleus” introduces the need to specifically transport mRNA and
proteins through the nuclear shell, and to dock capsids on the shell for genome packaging.
�...
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- Journal Article
Authors : Sahil, Mohammad (2023) - Bacterial transcription initiation mainly occurs via two diverse RNA polymerases, namely
70 and 54. While 70 polymerase transcribes housekeeping genes and does not require
any external activation to form transcriptionally competent open complex, the alternate
polymerase 54 require regulatory proteins, typically AAA+ ATPases, that aid in converting
the closed RNA polymerase complex to an active open state.1,2 External stimuli and
enviro...
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- Journal article
Authors : Manikandan, Amrutha (2023) - The AMP-activated protein kinase (AMPK) is known to be activated by the protein tyrosine
phosphatase non-receptor type 12 (PTP-PEST) under hypoxic conditions. This activation is
mediated by tyrosine dephosphorylation of the AMPKα subunit. However, the identity of the
phosphotyrosine residues remains unknown. In this study we first predicted the structure of
the complex of the AMPKα2 subunit and PTP-PEST catalytic domain using bioinformatics
too...
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- Journal article
Authors : Bageshwar, Umesh K. (2021) - The twin-arginine translocation (Tat) pathway transports folded proteins across energetic membranes.
Numerous Tat substrates contain co-factors that are inserted before transport with the assistance of redox
enzyme maturation proteins (REMPs), which bind to the signal peptide of precursor proteins. How signal
peptides are transferred from a REMP to a binding site on the Tat receptor complex remains unknown.
Since the signal peptide mediates both ...
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- Journal article
Authors : Park, Sang Ho (2021) - SARS-CoV-2 is the novel coronavirus that is the causative agent of COVID-19, a sometimes-lethal respiratory infection responsible for a world-wide pandemic. The envelope (E) protein, one of four structural proteins encoded in the viral genome, is a 75-residue integral membrane protein whose transmembrane domain exhibits ion channel activity and whose cytoplasmic domain participates in protein-protein interactions. These activities contribute to several aspects ...
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- Journal Article
Authors : Doulidis, Pavlos G. (2023) - Bloodwork is a widely used diagnostic tool in veterinary medicine, as diagnosis and therapeutic 15 interventions often rely on blood biomarkers. However, biomarkers available in veterinary medicine 16 often lack sensitivity or specificity. Mass spectrometry (MS)-based proteomics technology has been 17 extensively used in biological fluids and offers excellent potential for a more comprehensive 18 characterization of the plasma proteome in veterinary medicine. In this&#...
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- Journal Article
Authors : Henrion, André (2023) - Quantitative analysis depends on pure-substance primary calibrators with known mass fractions of impurity. Here, label-free quantification (LFQ) is being evaluated as a readily available, reliable method for determining the mass fraction of host-cell proteins (HCPs) in bioengineered proteins. For example, hemoglobin-A2 (HbA2) is being used as obtained through overexpression in E.coli. Two different materials had been produced: natural, and U-15N-labeled HbA2. For quantification&...
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- Journal article
Authors : Cannon, Kevin S. (2023) - processes such as signal transduction, membrane trafficking, and autophagy. Transient binding to the membrane
has a profound impact on protein function, serving to induce conformational changes and alter biochemical and
biophysical parameters by increasing the local concentration of factors and restricting diffusion to two dimensions. Despite the centrality of the membrane in serving as a template for cell biology, there are few reported highresolution stru...
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- Journal Article
Authors : Patel, Chinmaya Kumar (2023) - Protein aggregation and inactivation upon surface immobilization are major limiting factors for analytical applications in biotechnology related fields. Protein immobilization on solid surfaces often requires multi‐step surface passivation which is time consuming and inefficient. Herein, we have discovered that biomolecular condensates of biologically active human serum transferrin (Tf) can effectively prevent surface‐induced fibrillation and preserve the native‐like conformation of phase ...
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- Journal article
Authors : Phillips, Ian R. (2023) - Flavin-containing monooxygenase 5 (FMO5) is a member of the FMO family of proteins best
28 known for their roles in the detoxification of foreign chemicals and more recently in
29 endogenous metabolism. We have previously shown that Fmo5-/- mice display an age-related
30 lean phenotype, with much reduced weight gain from 20 weeks of age. The phenotype is
31 characterized by decreased fat deposition, lower plasma concentrations...
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- Journal Article
Authors : Ketaren, Natalia E. (2023) - SARS-CoV-2 has infected >40% of the world’s population (Collaborators 2022) resulting in
a devastating loss of life. As the SARS-CoV-2 pandemic enters its endemic phase (Meng,
Irwin et al. 2023, Pilz and Ioannidis 2023), multiple new variants continue to circulate. Since
its initial spread, the rapid adaptation of the virus to selective pressures continues to
produce variants of concern (VoC), of which the omicron variants presently ...
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- Journal article
Authors : Samant, Rahul S. (2023) - works in concert with co-chaperones to stabilize its client
proteins, which include multiple drivers of oncogenesis
and malignant progression. Pharmacologic inhibitors of
HSP90 have been observed to exert a wide range of
effects on the proteome, including depletion of client
proteins, induction of heat shock proteins, dissociation
of co-chaperones from HSP90, disruption of client protein
signaling networks, and recruitment of the protein
...
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- Journal article
Authors : Ganar, Ketan A. (2023) - Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich
saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid
transition is not yet understood. This study focuses on the phase transitions of a disordered glycine-
rich protein (GRP) that is prominent in tick saliva. We show that GRP undergoes liquid-liquid phase
separation via simple coacervatio...
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- Journal article
Authors : Shalev, Niva Tadmor (2023) - Determining seaweed protein concentration and the associated phenotype is critical for
food industries that require precise tools to moderate concentration fluctuations and
attenuate risks. Algal protein extraction and profiling have been widely investigated, but
content determination involves a costly, time-consuming, and high-energy, laboratory-based
fractionation technique. The present study examines the potential of field spectroscopy
technology as a precise, high-...
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- Journal article
Authors : Gegner, Hagen M. (2023) - Metabolomic and proteomic analyses of human plasma and serum samples harbour the power to advance our understanding of disease biology. Pre-analytical factors may contribute to variability and bias in the detection of analytes, especially when multiple labs are involved, caused by sample handling, processing time, and differing operating procedures. To better understand the impact of pre-analytical factors that are relevant to implement a unified proteomic and...
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