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Showing results 14 to 33 of 21507
  • OER000002841.pdf.jpg
  • Journal article


  • Authors : Franziska, Holz (2021)

  • Carbon Capture and Storage (CCS) might be a central technology to reach the decarbonisation goals of the European energy system. However, CCS deployment faces multiple economic, technological, and infrastructure challenges. Related literature tends to only focus on certain aspects of the CCS technology or to be limited to a particular sector perspective. In contrast, this paper presents a holistic modelling framework to analyse the longterm ...

  • OER000000845.pdf.jpg
  • Journal article


  • Authors : Ghomi, Fatemeh Ashari; Kittilä, Tiia; Welner, Ditte Hededam (2020)

  • UDP-dependent glycosyltransferases (UGTs) are enzymes that glycosylate a wide variety of natural products, thereby modifying their physico-chemical properties, i.e. solubility, stability, reactivity, and function. To successfully leverage the UGTs in biocatalytic processes, we need to be able to screen and characterise them in vitro, which requires efficient heterologous expression in amenable hosts, preferably Escherichia coli. However, many UGTs are insoluble when expressed in...

  • OER000002326.pdf.jpg
  • Journal Article


  • Authors : Daly, Leonard A. (2023)

  • Protein tyrosine sulfation (sY) is a post-translational modification (PTM) catalysed by Golgi-resident Tyrosyl Protein SulfoTransferases (TPSTs). Information on protein tyrosine sulfation is currently limited to ~50 human proteins with only a handful having verified sites of sulfation. The contribution of this chemical moiety for the regulation of biological processes, both inside and outside the cell, remains poorly defined, in large part due to analytica...

  • OER000000943.pdf.jpg
  • Journal article


  • Authors : Lorton, Benjamin M; Harijan, Rajesh K; Burgos, Emmanuel S (2020)

  • Using highly accurate quantitative binding analysis combined with high-resolution crystal structures of WDR5 in complex with unmodified (me0) and me1/me2s L-Arginine amino acids and in complex with H3R2me1 peptide, we provide a rigorous biochemical study of this important biological interaction. Despite modest structural differences at the binding interface, our study supports an interaction model regulated by a binary arginine methylation switch: H3R2me2a prevents int...

  • OER000002575.pdf.jpg
  • Journal article


  • Authors : Kim, C. (2023)

  • Orthosteric inhibition of kinases has been challenging due to the conserved active site architecture of kinases and emergence of resistance mutants. Simultaneous inhibition of distant orthosteric and allosteric sites, which we refer to as “double-drugging”, has recently been shown to be effective in overcoming drug resistance. However, detailed biophysical characterization of the cooperative nature between orthosteric and allosteric modulators has not be...

  • OER000002975.pdf.jpg
  • Journal article


  • Authors : Raeburn, Candice B. (2021)

  • An extensive network of chaperones and other proteins maintain protein homeostasis and guard against inappropriate protein aggregation that is a hallmark of neurodegenerative diseases. Using a fluorescence resonance energy-based biosensor that simultaneously reports on intact cellular chaperone holdase activity and detrimental aggregation propensity, we investigated the buffering capacity of the systems managing protein homeostasis in the nucleus of the human cell l...

  • OER000002116.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : C.H., Swaroop (2020)

  • Python is probably one of the few programming languages which is both simple and powerful. This is good for beginners as well as for experts, and more importantly, is fun to program with. This book aims to help you learn this wonderful language and show how to get things done quickly and painlessly - in effect 'The Anti-venom to your programming problems'.

  • OER000003222.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : Richard, Bondi (2019)

  • Trong một tổ chức SRE, các nhóm thường phát triển các công cụ và quy trình tự động hóa rất khác nhau để hoàn thành các nhiệm vụ tương tự. Một số điều này có thể được giải thích bằng phần mềm mà chúng hỗ trợ: các hệ thống khác nhau yêu cầu các giải pháp có độ tin cậy khác nhau. Nhưng nhiều nhiệm vụ SRE về cơ bản là giống nhau trên tất cả các phần mềm: biên dịch, xây dựng, triể...

  • OER000000266.pdf.jpg
  • Periodicals (Báo – Tạp chí)


  • Authors : Strzelecka, Dominika; Smietanski, Miroslaw (2020)

  • Chemical modifications enable preparation of mRNAs with augmented stability and translational activity. In this study, we explored how chemical modifications of 5’,3’-phosphodiester bonds in the mRNA body and polyA tail influence the biological properties of eukaryotic mRNA. To obtain modified and unmodified in vitro transcribed mRNAs, we used ATP and ATP analogues modified at the α-phosphate (containing either O-to-S or O-to-BH3 substitutions) and three different ...

  • OER000000265.pdf.jpg
  • Periodicals (Báo – Tạp chí)


  • Authors : Ruggiero, Melissa J; Malhotra, Shipra (2020)

  • Conventionally, most amino acid substitutions at important protein positions are expected to abolish function. However, in several soluble-globular proteins, we identified a class of non-conserved positions for which various substitutions produced progressive functional changes; we consider these evolutionary “rheostats”. Here, we report a strong rheostat position in the integral membrane protein, Na+/taurocholate cotransporting polypeptide (NTCP), at the site of a pharmacologically-...

  • OER000002990.pdf.jpg
  • Journal article


  • Authors : Soubeyrand, Sébastien (2021)

  • Fibronectin (FN1) is an essential regulator of homodynamic processes and tissue remodeling which has been proposed to contribute to atherosclerosis. Moreover, recent large scale genome wide association studies have linked common genetic variants within the FN1 gene to coronary artery disease (CAD) risk. Genome-wide Association Studies (GWAS) have identified hundreds of common single nucleotide polymorphisms (SNPs) that associate with cardiovascular disease (CA...

  • OER000004042.pdf.jpg
  • Journal article


  • Authors : Jensen, Dorte B. Bekker; Val, Ana Martínez del; Steigerwald, Sophia (2019)

  • State-of-the-art proteomics-grade mass spectrometers can measure peptide precursors and their fragments with ppm mass accuracy at sequencing speeds of tens of peptides per second with attomolar sensitivity. Here we describe a compact and robust quadrupole-orbitrap mass spectrometer equipped with a front-end High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Interface. The performance of the Orbitrap Exploris 480 mass spectrometer is evaluated in data-dependent ...

  • OER000002831.pdf.jpg
  • Journal article


  • Authors : Nicholas, Nixon Opiyo (2019)

  • Sub-Saharan Africa continues to suffer energy poverty due to low grid expansion rates necessitated by low economic activities in those regions, sparse population distribution coupled with low household load demands, and insufficient power generation. On the other hand, small solar power microgeneration systems have emerged as potential alternatives to grid electrifications, enabling households to make modest investments into their power systems, and to&#x...

  • OER000002629.pdf.jpg
  • Journal article


  • Authors : George, Amy L. (2023)

  • Thermal proteome profiling (TPP) provides a powerful approach to studying proteome-wide interactions of small therapeutic molecules and their target and off-target proteins, complementing phenotypic-based drug screens. Detecting differences in thermal stability due to target engagement requires high quantitative accuracy and consistent detection. Isobaric tandem mass tags (TMT) are used to multiplex samples and increase quantification precision in TPP analysis by data-dependent acqui...

  • OER000004038.pdf.jpg
  • Journal article


  • Authors : Neupane, Ritam; Pisareva, Vera P; Rodríguez, Carlos F (2019)

  • RNA viruses are pervasive entities in the biosphere with significant impact in human health and economically important livestock. As strict cellular parasites, RNA viruses abuse host resources, redirecting them towards viral replication needs. Taking control of the cellular apparatus for protein production is a requirement for virus progression and diverse strategies of cellular mimicry and/or ribosome hijacking evolved to ensure this control. Especially in comple...

  • OER000000412.pdf.jpg
  • Journal article


  • Authors : Jong, Luitzen de; Roseboom, Winfried; Kramer, Gertjan (2020)

  • In vivo chemical cross-linking combined with LCMSMS of digested extracts (in vivo CX-MS) can reveal stable and dynamic protein-protein interactions at a proteome wide-scale and at the peptide level. In vivo CX-MS requires a membrane permeable and cleavable cross-linker that enables isolation of target peptides and a fast and sensitive search engine to identify the linked peptides. Here we explore the use of the search engine pLink 2 for analysi...

  • OER000002951.pdf.jpg
  • Journal article


  • Authors : Sailer, Carolin (2023)

  • Eukaryotic ribosome biogenesis is facilitated and regulated by numerous ribosome biogenesis factors (RBFs). High-resolution cryo-EM maps have defined the molecular interactions of RBFs during maturation, but many transient and dynamic interactions, particularly during early assembly, remain uncharacterized. Using quantitative proteomics and crosslinking coupled to mass spectrometry (XL-MS) data from a extensive set of pre-ribosomal particles, we derived a comprehensive...

  • OER000002840.pdf.jpg
  • Journal article


  • Authors : Paolo, Falbo (2021)

  • Due to recent technical progress, battery energy storages are becoming a viable option in the power sector. Their optimal operational management focuses on load shift and shaving of price spikes. However, this requires optimally responding to electricity demand, intermittent generation, and volatile electricity prices. More importantly, such optimization must take into account the so-called deep discharge costs, which have a significant impact on bat...

  • OER000002618.pdf.jpg
  • Journal article


  • Authors : Melo, Eduardo P. (2023)

  • Protein disulfide isomerases (PDIs) are a family of molecular chaperones resident in the endoplasmic reticulum (ER) emerging as important factors in disease. In addition to an holdase function, some members catalyse disulfide bond formation and isomerization, a crucial step for native folding and prevention of aggregation of misfolded proteins. PDIs are characterized by a modular arrangement of thioredoxin-like domains, with the canonical, first identified PDIA1, ...