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Showing results 5081 to 5100 of 22767
  • OER000004055.pdf.jpg
  • Journal article

  • Ibotenic acid biosynthesis in the fly agaric is initiated by glutamate hydroxylation

    • Authors : Obermaier, Sebastian; Müller, Michael (2019)

  • The fly agaric, Amanita muscaria, is widely known for its content of the psychoactive metabolites ibotenic acid and muscimol. 50 years ago, their biosynthesis was hypothesized to start with 3-hydroxyglutamate. Here, we build on this hypothesis by the identification and recombinant production of a glutamate hydroxylase from A. muscaria. The corresponding gene is surrounded by six other genes, which we link to ibotenic acid production using recent genetic&...
  • OER000003007.pdf.jpg
  • Journal article

  • Identification and assessment of cardiolipin interactions with E. coli inner membrane proteins

    • Authors : Corey, Robin A. (2021)

  • Integral membrane proteins are localised and/or regulated by lipids present in the surrounding bilayer. Whilst bacteria such as E. coli have relatively simple membranes when compared to eukaryotic cells, there is ample evidence that many bacterial proteins bind to specific lipids, especially the anionic lipid cardiolipin. Here, we apply molecular dynamics simulations to assess lipid binding to 42 different E. coli inner membrane proteins. O...
  • OER000003033.pdf.jpg
  • Journal article

  • Identification and characterization of novel plasma proteins in drug resistant HIV/AIDS patients by SWATH-MS

    • Authors : Barik, Sushanta Kumar (2021)

  • Acquired immunodeficiency syndrome is one of the most important diseases caused by human immunodeficiency virus. Understanding its molecular pathogenesis is essential to manage the disease at the population level. In this study, a quantitative analysis of plasma proteins was carried out in drug resistant and drug respondent patients using the SWATH-MS. Sequential window acquisition of all theoretical mass spectra (SWATH-MS) is a prime technique to seek...
  • OER000002503.pdf.jpg
  • Journal article

  • Identification of a new Corin atrial natriuretic peptide-converting enzyme substrate: Agouti-signaling protein (ASIP)

    • Authors : MacDonald, Bryan T.  (2023)

  • Corin is a transmembrane tethered enzyme best known for processing the hormone atrial natriuretic peptide (ANP) in cardiomyocytes to control electrolyte balance and blood pressure. Loss of function mutations in Corin prevent ANP processing and lead to hypertension. Curiously, Corin loss of function variants also result in lighter coat color pigmentation in multiple species. Corin pigmentation effects are dependent on a functional Agouti locus encoding the ago...
  • OER000002564.pdf.jpg
  • Journal article

  • Identification of a Novel PARP14 Site Motif and Glycohydrolase Specificity Using TLC-MALDI-TOF

    • Authors : Javed, Zeeshan (2023)

  • Transfer of ADP-ribose (ADPr) from nicotinamide adenine dinucleotide (NAD+) to target proteins is mediated by a class of human poly-ADP-ribose polymerases, PARPs, and removal of ADPr is catalyzed by a family of glycohydrolases. Although thousands of potential ADPr modification sites have been identified using high-throughput mass-spectrometry, relatively little is known about sequence specificity encoded near the modification site. Herein, we present a matrix-assisted ...
  • OER000004054.pdf.jpg
  • Journal article

  • Identification of hemicatenane-specific binding proteins by fractionation of Hela nuclei extracts

    • Authors : Rouis, Oumayma; Broussard, Cédric; Guillonneau, François (2019)

  • DNA hemicatenanes (HCs) are DNA structures in which one strand of a double stranded helix passes through the two strands of another double stranded DNA. Frequently mentioned as DNA replication, recombination and repair intermediates, they have been proposed to participate in the spatial organization of chromosomes and in the regulation of gene expression. To explore potential roles of HCs in genome metabolism, proteins capable of binding specifically HCs...
  • OER000000830.pdf.jpg
  • Journal article

  • Identification of novel glycosylation events on human serum-derived Factor IX

    • Authors : Pegg, Cassandra L; Zacchi, Lucia F; Recinos, Dinora Roche (2020)

  • Human Factor IX is a highly post-translationally modified protein that is an important clotting factor in the blood coagulation cascade. Functional deficiencies in Factor IX result in the bleeding disorder haemophilia B, which is treated with plasma-derived or recombinant Factor IX concentrates. Here, we investigated the post-translational modifications of human serum-derived Factor IX and report previously undescribed O-linked monosaccharide compositions at serine 141 and&...
  • OER000000840.pdf.jpg
  • Journal article

  • Identification of PCNA interacting protein motifs in human DNA polymerase delta

    • Authors : Khandagale, Prashant; Thakur, Shweta; Acharya, Narottam (2020)

  • DNA polymerase delta (Polδ) is a highly processive essential replicative DNA polymerase. In humans, Polδ holoenzyme consists of p125, p50, p68, and p12 subunits and recently, we have shown that p12 exists as a dimer. Extensive biochemical studies suggest that all the subunits of Polδ interact with the processivity factor proliferating cell nuclear antigen (PCNA) to carry out a pivotal role in genomic DNA replication. While PCNA interaction protein&#...
  • OER000002436.pdf.jpg
  • Ebooks (Sách điện tử)

  • Identification of proteins that bind extracellular microRNAs secreted by the parasitic nematode Trichinella spiralis

    • Authors : Brown, A. (2023)

  • Small non-coding RNAs such as microRNAs (miRNAs) are conserved across eukaryotes and play key roles in regulating gene expression. In many organisms, miRNAs are also secreted from cells, often encased within vesicles such as exosomes and sometimes extravesicular. The mechanisms of miRNA secretion, how they are stabilised outside of cells and their functional importance are poorly understood. Recently we characterised the parasitic nematode Trichinella spiralis as&...
  • OER000002291.pdf.jpg
  • Journal Article

  • Identification of putative druggable pockets in PRL3, a significant oncology target, using in silico analysis

    • Authors : Bennett, Grace M. (2023)

  • Aberrant cellular phosphorylation is a hallmark of several diseases including inflammation and cancers (1–3). Protein phosphorylation is a post-translational modification that can act as a switch to regulate biochemical pathways and is regulated by the concerted action of two classes of enzymes: kinases and phosphatases. As such, kinases and phosphatases present as significant potential clinical molecular targets. To date, several kinase inhibitors have&#...
  • OER000002456.pdf.jpg
  • Journal Article

  • Identification of the bacteriophage nucleus protein interaction network

    • Authors : Enustun, Eray (2023)

  • In the arms race between bacteria and bacteriophages (phages), some large-genome jumbo phages have evolved a protein shell that encloses their replicating genome to protect it against DNA-targeting immune factors. By segregating the genome from the host cytoplasm, however, the “phage nucleus” introduces the need to specifically transport mRNA and proteins through the nuclear shell, and to dock capsids on the shell for genome packaging. �...
  • OER000002443.pdf.jpg
  • Ebooks (Sách điện tử)

  • Identification of the intracellular protein targets of a bio-active clickable half-sandwich iridium complex by chemical proteomics

    • Authors : Ramos, Robin (2023)

  • Identification of intracellular targets of anticancer drug candidates provides key information on their mechanism of action. Exploiting the ability of the anticancer (C^N)-chelated half-sandwich iridium(III) complexes to covalently bind proteins, click chemistry with a bioorthogonal azido probe was used to localize a phenyloxazoline-chelated iridium complex within cells and profile its interactome at the proteome-wide scale. Proteins involved in protein folding and...
  • OER000002929.pdf.jpg
  • Journal article

  • Identification of ubiquitin variants that inhibit the E2 ubiquitin conjugating enzyme, Ube2k

    • Authors : Middleton, A.J. (2021)

  • Transfer of ubiquitin to substrate proteins regulates most processes in eukaryotic cells. E2 enzymes are a central component of the ubiquitin machinery, and generally determine the type of ubiquitin signal generated and thus the ultimate fate of substrate proteins. The E2, Ube2k, specifically builds degradative ubiquitin chains on diverse substrates. Here we have identified protein-based reagents, called ubiquitin variants (UbVs), that ...
  • OER000002332.pdf.jpg
  • Journal Article

  • Identifying Selectivity Filters in Protein Biosensor for Ligand Screening

    • Authors : Sahil, Mohammad (2023)

  • Bacterial transcription initiation mainly occurs via two diverse RNA polymerases, namely 70 and 54. While 70 polymerase transcribes housekeeping genes and does not require any external activation to form transcriptionally competent open complex, the alternate polymerase 54 require regulatory proteins, typically AAA+ ATPases, that aid in converting the closed RNA polymerase complex to an active open state.1,2 External stimuli and enviro...
  • OER000001432.pdf.jpg
  • Ebooks (Sách điện tử)

  • IEA International Computer and Information Literacy Study 2018 Assessment Framework

    • Authors : Fraillon, Julian (2019)

  • This open-access book presents the assessment framework for IEA's International Computer an Information Literacy Study (ICILS) 2018, which is designed to assess how well students are prepared for study, work and life in a digital world. The study measures international differences in students' computer and information literacy (CIL): their ability to use computers to investigate, create, participate and communicate at home, at school, in the workplace ...
  • OER000001362.pdf.jpg
  • Ebooks (Sách điện tử)

  • IFLA Public Library Service Guidelines

    • Authors : Koontz, Christie; Gubbin, Barbara (2010)

  • The public library is the prime community access point designed to respond to a multitude of ever-changing information needs. These guidelines are framed to provide assistance to library and information professionals in most situations. They assist to better develop effective services, relevant collections, and accessible formats within the context and requirements of the local community. In this exciting and complex information world it is important for prof...