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Showing results 14240 to 14259 of 21273
  • OER000002708.pdf.jpg
  • Journal article


  • Authors : Dhingra, Yukti (2023)

  • Adaptation in CRISPR-Cas systems immunizes bacteria and archaea against mobile genetic 10 elements. In many DNA-targeting systems, the Cas4-Cas1-Cas2 complex is required for selection 11 and processing of DNA segments containing PAM sequences, prior to integration of these 12 “prespacer” substrates as spacers in the CRISPR array. We determined cryo-EM structures of the 13 Cas4-Cas1-Cas2 adaptation complex from the type I-C system that encodes st...

  • OER000000866.pdf.jpg
  • Journal article


  • Authors : Karvelis, Tautvydas; Bigelyte, Greta; Young, Joshua K (2020)

  • In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer unprecedented flexibility, however, more compact versions would simplify delivery and extend application. Here, we present a collection of 10 exceptionally compact (422-603 amino acids) CRISPR-Cas nucleases that recognize&#x...

  • OER000002702.pdf.jpg
  • Journal article


  • Authors : Kreimer, Simion (2023)

  • Proteomic analysis on the scale that captures population and biological heterogeneity over hundreds to thousands of samples requires rapid mass spectrometry methods which maximize instrument utilization (IU) and proteome coverage while maintaining precise and reproducible quantification. To achieve this, a short liquid chromatography gradient paired to rapid mass spectrometry data acquisition can be used to reproducibly profile a moderate set of analytes. ...

  • OER000003017.pdf.jpg
  • Journal article


  • Authors : Ruther, Joachim (2021)

  • Fatty acids are crucial primary metabolites for virtually any creature on earth. Therefore, most organisms do not rely exclusively on nutritional supply with fatty acids but have the ability to synthesize fatty acids and triacylglycerides de novo from carbohydrates, a process called lipogenesis. The ubiquity of lipogenesis has been questioned by a series of studies reporting that many parasitic wasps (parasitoids) do not accumulate lipid mass despite hav...

  • OER000001616.pdf.jpg
  • Book


  • Authors : - (2020)

  • This first open access volume of the handbook series contains articles on the standard model of particle physics, both from the theoretical and experimental perspective. It also covers related topics, such as heavy-ion physics, neutrino physics and searches for new physics beyond the standard model.

  • OER000001618.pdf.jpg
  • Book


  • Authors : - (2020)

  • This third open access volume of the handbook series deals with accelerator physics, design, technology and operations, as well as with beam optics, dynamics and diagnostics. A joint CERN-Springer initiative, the “Particle Physics Reference Library” provides revised and updated contributions based on previously published material in the well-known Landolt-Boernstein series on particle physics, accelerators and detectors (volumes 21A,B1,B2,C), which took stock of the field&#...

  • OER000000742.pdf.jpg
  • Journal article


  • Authors : Lobel, Joseph H; Gross, John D (2020)

  • Pat1 promotes the activation and assembly of multiple proteins during mRNA decay. After deadenylation, the Pat1/Lsm1-7 complex binds to transcripts containing oligo(A) tails, which can be modified by the addition of several terminal uridine residues. Pat1 enhances Lsm1-7 binding to the 3’ end, but it is unknown how this interaction is influenced by nucleotide composition. Here we examine Pat1/Lsm1-7 binding to a series of oligoribonucleotides containing&...

  • OER000002196.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : Schuster, Michael (-)

  • What are Patents, Trademarks, Servicemarks, and Copyrights Some people confuse patents, copyrights, and trademarks. Although there may be similarities among these kinds of intellectual property protection, they are different and serve different purposes. Policy Behind Patent Laws: Congress created the patent system under a constitutional mandate, stating that such a system can be enacted “To promote the Progress of Science and useful Arts.” This promotion of ...

  • OER000002468.pdf.jpg
  • Journal Article


  • Authors : Browning, Kaitlyn R. (2023)

  • Pathogenic bacteria and their eukaryotic hosts are in a constant arms race. Hosts have numerous defense mechanisms at their disposal that not only challenge the bacterial invaders, but have the potential to disrupt molecular transactions along the bacterial chromosome. However, it is unclear how the host impacts association of proteins with the bacterial chromosome at the molecular level during infection. This is partially due to the lack of a ...

  • OER000000017.pdf.jpg
  • Journal article


  • Authors : Hans-Karl, Bartholdsen (2019)

  • Describes three possible pathways for the transformation of the German energy system until 2050. The scenarios take into account current climate politics on a global, European, and German level and also include different demand projections, technological trends and resource prices. The model includes the sectors power, heat, and transportation and works on a Federal State level. For the analysis, the linear cost-optimizing Global Energy System Model (GE...

  • OER000001663.pdf.jpg
  • Book


  • Authors : Frühwirth, Rudolf; Strandlie, Are (2021)

  • This open access book is a comprehensive review of the methods and algorithms that are used in the reconstruction of events recorded by past, running and planned experiments at particle accelerators such as the LHC, SuperKEKB and FAIR. The main topics are pattern recognition for track and vertex finding, solving the equations of motion by analytical or numerical methods, treatment of material effects such as multiple Coulomb scattering and ener...

  • OER000001452.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : Bernstein, David (2022)

  • Programming patterns are solutions to problems that require the creation of a small fragment of code that will be part of a larger program. Hence, this book is about teaching you how to write such fragments of code. However, it is not about teaching you the syntax of the statements in the fragments, it assumes that you already know the syntax. Instead, it is about finding solutions to problems that arise when first learning&...

  • OER000003218.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : Paul A., Carter (2019)

  • Cuốn sách này có nội dung bao quát về lắp ráp giao tiếp và mã C, do đó có thể được các lập trình viên C muốn tìm hiểu về cách hoạt động của C. Tất cả các ví dụ đều sử dụng trình biên dịch NASM (Netwide) miễn phí. Hướng dẫn này chỉ đề cập đến việc lập trình ở chế độ được bảo vệ 32 bit và yêu cầu trình biên dịch chế độ được bảo vệ 32 bit. Có thể sử dụng sách và&#...

  • OER000000883.pdf.jpg
  • Journal article


  • Authors : Kulkarni, Dhananjaya S; Owens, Shannon; Honda, Masayoshi (2020)

  • During meiosis, crossover recombination connects homologous chromosomes to direct their accurate segregation1. Defects in crossing over cause infertility, miscarriage and congenital disease. Accordingly, each pair of chromosomes attains at least one crossover through processes that designate and then implement crossing over with high efficiency2. At the DNA level, crossing over is implemented through the formation and biased resolution of double-Holliday Junction intermediates3–5.&#x...

  • OER000002197.pdf.jpg
  • Ebooks (Sách điện tử)


  • Authors : rsinha, Rajeeva (2018)

  • Financing old age or pensions is a challenge for individuals and societies. Longevity has been rising across the world. More more relevant to retirement, life expectancy at age 60 has also been on the increase according to the World Health Organization. From a finance perspective there is another reason why we need to study pensions. Much of what we discuss in standard finance curriculum assumes an individual decision maker motivated by&#x...

  • OER000000212.pdf.jpg
  • Journal article


  • Authors : Mazurek, Łukasz; Ghilarov, Dmitry; Michalczyk, Elizabeth (2020)

  • Activities of two such proteins, QnrB1 and AlbG in vitro. Each of them provided specific protection against its cognate toxin (fluoroquinolone or albicidin), which strictly required ATP hydrolysis by gyrase. Through a combination of fluorescence anisotropy, pull-downs and photocrosslinking we show that QnrB1 binds to the GyrB protein. We further probed the QnrB1 binding site using site-specific incorporation of a photoreactive amino acid and mapped strong and...