OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường (1401)



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Danh sách tài liệu trong bộ sưu tập

Danh sách tài liệu trong bộ sưu tập (Sắp xếp theo "Ngày nhập " với thứ tự "Giảm dần "): Hiển thị 1-20 trong tổng số 1401 tài liệu

  • OER000004019.pdf.jpg
  • Journal article


  • Tác giả : Williams, Daniel E; Le, Sarah N; Hoke, David E (2019)

  • Thyroid peroxidase (TPO) is a critical membrane-bound enzyme involved in the biosynthesis of multiple thyroid hormones, and is a major autoantigen in autoimmune thyroid diseases such as Graves’ disease and Hashimoto’s thyroiditis. Here we report the biophysical and structural characterisation of two novel TPO constructs containing only the ectodomain of TPO and lacking the propeptide. Both constructs were enzymatically active and able to bind the patient-derived&#...

  • OER000004021.pdf.jpg
  • Journal article


  • Tác giả : Palka, Christina; Forino, Nicholas M; Hentschel, Jendrik (2019)

  • Telomeres safeguard the genome by suppressing illicit DNA damage responses at chromosome termini. In order to compensate for incomplete DNA replication at telomeres, most continually dividing cells, including many cancers, express the telomerase ribonucleoprotein (RNP) complex. Telomerase maintains telomere length by catalyzing de novo synthesis of short DNA repeats using an internal telomerase RNA (TR) template. TRs from diverse species harbor structurally conserved domain...

  • OER000004018.pdf.jpg
  • Journal article


  • Tác giả : Zhou, Fengyan; Cao, Guozheng; Dai, Songjun (2019)

  • By alternative splicing, Drosophila Down syndrome cell adhesion molecule (Dscam1) encodes tens of thousands of proteins required for establishing neural circuits, while Chelicerata encodes a family of ∼ 100 shortened Dscam (sDscam) isoforms via alternative promoters. We report that Dscam isoforms interact promiscuously in cis to generate a vast repertoire of combinatorial homophilic recognition specificities in Chelicerata. Specifically, sDscams formed high order cis-multimers&#...

  • OER000004017.pdf.jpg
  • Journal article


  • Tác giả : A, Cezanne; J, Lauer; A, Solomatina (2019)

  • Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effector binding, generating a positive feedback of GTPase activation and membrane recruitment. Here, we reconstituted the patterning of the small GTPase Rab5 and its GEF/effector complex Rabex5/Rabaptin5 on supported lipid bilaye...

  • OER000004016.pdf.jpg
  • Journal article


  • Tác giả : Troein, Carl; Siregar, Syahril; Beeck, Michiel Op De (2019)

  • Modern vibrational spectroscopy techniques enable rapid collection of thousands of spectra in a single hyperspectral image, allowing researchers to resolve spatially heterogeneous samples down to a resolution of a few μm. A number of algorithms have been developed to correct for effects such as atmospheric absorption, light scattering by cellular structures and varying baseline levels. Following such preprocessing, spectra are commonly decomposed and clustered to ...

  • OER000004015.pdf.jpg
  • Journal article


  • Tác giả : Prigozhin, Daniil M; Albecka, Anna; Douse, Christopher H (2019)

  • Transcription of integrated DNA from viruses or transposable elements is tightly regulated to prevent pathogenesis. The Human Silencing Hub (HUSH), composed of Periphilin, TASOR and MPP8, silences transcriptionally active viral and endogenous transgenes. HUSH recruits effectors that alter the epigenetic landscape and chromatin structure, but how HUSH recognizes target loci and represses their expression remains unclear. We identify the physicochemical properties of Periphilin&#x...

  • OER000004014.pdf.jpg
  • Journal article


  • Tác giả : Baretić, Domagoj; Bedford, Michael Jenkyn; Aria, Valentina (2019)

  • The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase, orchestrates chromosome replication. Multiple factors associate directly with CMG including Ctf4 and the heterotrimeric fork protection complex (Csm3/Tof1 and Mrc1), that have important roles including aiding normal replication rates and stabilizing stalled forks. How these proteins interface with CMG to execute these functions is poorly understood. Here we present 3-3.5 Å resolution cryo-EM struc...

  • OER000004013.pdf.jpg
  • Journal article


  • Tác giả : Rojo, Noemi Jiménez; Leonetti, Manuel D; Zoni, Valeria (2019)

  • Sphingolipids have been shown to play important roles in physiology and cell biology, but a systematic examination of their functions is lacking. We performed a genome-wide CRISPRi screen in sphingolipid-depleted cells and identified hypersensitive mutants in genes of membrane trafficking and lipid biosynthesis, including ether lipid synthesis. Systematic lipidomic analysis showed a coordinate regulation of ether lipids with sphingolipids, where depletion of one of the...

  • OER000004012.pdf.jpg
  • Journal article


  • Tác giả : Giglio, M.L; Ituarte, S; Milesi, V (2019)

  • The Membrane Attack Complex-Perforin (MACPF) family is ubiquitously found in all kingdoms. They have diverse cellular roles but MACPF but pore-forming toxic function are very rare in animals. Here we present the structure of PmPV2, a MACPF toxin from the poisonous apple snail eggs, that can affect the digestive and nervous systems of potential predators. We report the three-dimensional structure of PmPV2, at 15 Å resolution determined by negative&#x...

  • OER000004011.pdf.jpg
  • Journal article


  • Tác giả : Winkelman, Jared T; Pukhrambam, Chirangini; Vvedenskaya, Irina O (2019)

  • Pausing by RNA polymerase (RNAP) during transcription elongation, in which a translocating RNAP uses a “stepping” mechanism, has been studied extensively, but pausing by RNAP during initial transcription, in which a promoter-anchored RNAP uses a “scrunching” mechanism, has not. We report a method that directly defines RNAP-active-center position relative to DNA in vivo with single-nucleotide resolution (XACT-seq; crosslink-between-active-center-and-template sequencing). We apply this...

  • OER000004010.pdf.jpg
  • Journal article


  • Tác giả : Tan, Yong Zi; Zhang, Lei; Rodrigues, José (-)

  • Mycobacterium tuberculosis causes tuberculosis, a disease that kills over one million people each year. Its cell envelope is a common antibiotic target and has a unique structure due, in part, to two lipidated polysaccharides – arabinogalactan and lipoarabinomannan. Arabinofuranosyltransferase D (AftD) is an essential enzyme involved in assembling these glycolipids. We present the 2.9 Å resolution structure of M. abscessus AftD determined by single particle cryo-e...

  • OER000004009.pdf.jpg
  • Journal article


  • Tác giả : Schakowski, Kai Melvin; Linders, Jürgen; Ferenz, Katja Bettina; Kirsch, Michael (2019)

  • Bovine serum albumin (BSA)-coated haemoglobin (Hb)-microcapsules prepared by co-precipitation of Hb and MnCO3 may present an alternative type of artificial blood substitute. Prepared microcapsules were analysed by Scanning electron microscopy (SEM) and Respirometry, cytotoxicity was evaluated by addition of microcapsules to murine fibroblast-derived cell line L929 (American Type Culture Collection, NCTC clone 929 of strain L). The capsules come along with a mean diameter&#x...

  • OER000004008.pdf.jpg
  • Journal article


  • Tác giả : Moretto, Fabien; Wood, N. Ezgi; Chia, Minghao (2019)

  • Many long noncoding RNAs (lncRNAs) act in cis through transcription-coupled chromatin alterations that drive changes in local gene expression. How some cis-acting lncRNAs promote and others repress gene expression remains poorly understood. Here we report that in S. cerevisiae transcription levels of the lncRNA IRT2, located upstream in the promoter of the inducer of meiosis gene, regulate opposing chromatin and transcription states. Low IRT2 transcription display...

  • OER000004006.pdf.jpg
  • Journal article


  • Tác giả : Galloway, Alison; Atrih, Abdelmadjid; Grzela, Renata (2019)

  • Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the triphosphate at the 5’ end. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap structures which protect RNA from degradation and recruit proteins involved in RNA processing and translation. Research demonstrating that the cap methyltransferases are regulated has generated...

  • OER000004005.pdf.jpg
  • Journal article


  • Tác giả : Warnasooriya, Chandani; Glasser, Eliezra; Feeney, Callen F (2019)

  • An essential heterodimer of the U2AF1 and U2AF2 pre-mRNA splicing factors nucleates spliceosome assembly at polypyrimidine (Py) signals preceding the major class of 3ʹ splice sites. Among myelodysplastic syndromes (MDS), U2AF1 frequently acquires an S34F-encoding mutation. The influence of the U2AF1 subunit and its S34F mutation on the U2AF2 conformations remains unknown. Here, we employ single molecule Förster resonance energy transfer (FRET) to determine the inf...

  • OER000004003.pdf.jpg
  • Journal article


  • Tác giả : Modenutti, Carlos P; Capurro, Juan I. Blanco; Ibba, Roberta (2020)

  • UDP-glucose:glycoprotein glucosyltransferase (UGGT) is the only known glycoprotein folding quality control checkpoint in the eukaryotic glycoprotein secretory pathway. When the enzyme detects a misfolded glycoprotein in the Endoplasmic Reticulum (ER), it dispatches it for ER retention by re-glucosylating it on one of its N-linked glycans. Recent crystal structures of a fungal UGGT have suggested the enzyme is conformationally mobile. Here, a negative stain electron mic...

  • OER000001575.pdf.jpg
  • Journal article


  • Tác giả : Mrozowich, Tyler; Henrickson, Amy; Demeler, Borries (2020)

  • Viral infections are responsible for numerous deaths worldwide. Flaviviruses, which contain RNA as their genetic material, are one of the most pathogenic families of viruses. There is an increasing amount of evidence suggesting that their 5’ and 3’ non-coding terminal regions are critical for their survival. In this study, the 5’ and 3’ terminal regions of Murray Valley Encephalitis and Powassan virus were examined using biophysical and computational...

  • OER000001602.pdf.jpg
  • Journal article


  • Tác giả : Madlung, Johannes; Bansal, Prashali; Schaaf, Kristina (2020)

  • During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and ...