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  • Journal article

  • Authors : Hsia, Oliver (2023)

  • Targeted protein degradation is a drug modality represented by compounds that recruit a target to an E3 ubiquitin ligase to promote target ubiquitination and proteasomal degradation. Historically, the field distinguishes monovalent degraders from bifunctional degraders (PROTACs) that connect target and ligase via separate binding ligands joined via a linker1-4. Here, we elucidate the mechanism of action of a PROTAC-like degrader of the transcription...

  • Journal article

  • Authors : Kozome, Dan (2023)

  • Loops are small secondary structural elements that play a crucial role in the emergence of new 14 enzyme functions. However, our understanding of loop functions is mainly limited to the 15 catalytic loops. To understand the function of remote loops in enzymes, we studied Glycoside 16 hydrolase family 19 (GH19) chitinase - an essential enzyme family for pathogen degradation 17 in plants. By revealing the evolutionary history and loops appearance...

  • Journal article

  • Authors : Dauzier, N. Lobato- (2023)

  • Complex organisms perceive their surroundings with sensory neurons which encode physical stimuli into spikes of electrical activities. The past decade has seen reports of DNA-based chemical neurons that mimic artificial neural networks with chemical reactions. Yet, they lack the physical sensing and temporal coding of sensory biological neurons. Here we report a thermosensory chemical neuron based on DNA and enzymes that spikes with chemical act...

  • Journal article

  • Authors : Roy, Rahul (2023)

  • Amplification-based qPCR provides accurate and sensitive nucleic acid quantification. However, the requirement of temperature cycling and real-time monitoring limits its translation to different settings. Here, we adapted isothermal Recombinase Polymerase Amplification (RPA) reaction to develop a semi-quantitative method that relies on final amplicon yield to estimate initial target nucleic acid copy number. To achieve this, we developed a phenomenological model&#...

  • Journal Article

  • Authors : Elena-Real, Carlos A.  (2023)

  • NMR studies of large biomolecular machines and highly repetitive proteins remain challenging due to the difficulty of assigning signals to individual nuclei. Here, we present an efficient strategy to address this challenge by engineering a Pyrococcus horikoshii tRNA/alanyl-tRNA synthetase pair that enables the incorporation of up to three isotopically labeled alanine residues in a site-specific manner using in vitro protein expression. We have d...