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  • Journal Article

  • A robust method for measuring aminoacylation through tRNA-Seq

    • Authors : Davidsen, Kristian (2023)

  • Current methods to quantify the fraction of aminoacylated tRNAs, also known as the 8 tRNA charge, are limited by issues with either low throughput, precision, and/or accuracy. Here, we 9 present an optimized charge tRNA-Seq method that combines previous developments with newly 10 described approaches to establish a protocol for precise and accurate tRNA charge measurements. 11 We verify that this protocol provides robust quantification ...
  • Journal Article

  • An engineered biosensor enables dynamic aspartate measurements in living cells

    • Authors : Davidsen, Kristian (2023)

  • Intracellular levels of the amino acid aspartate are responsive to changes in metabolism in mammalian cells and can correspondingly alter cell function, highlighting the need for robust tools to measure aspartate abundance. However, comprehensive understanding of aspartate metabolism has been limited by the throughput, cost, and static nature of the mass spectrometry based measurements that are typically employed to measure aspartate levels.&...