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  • Journal article

  • Sphingolipid-Induced Programmed Cell Death Is a Salicylic Acid and EDS1-Dependent Phenotype in Arabidopsis

    • Authors : König, Stefanie (2021)

  • Ceramides and long chain bases (LCBs) are plant sphingolipids involved in the induction of plant programmed cell death (PCD). The fatty acid hydroxylase mutant fah1 fah2 exhibits high ceramide levels and moderately elevated LCB levels. Salicylic acid (SA) is strongly induced in these mutants, but no cell death is visible. To determine the effect of ceramides with different chain lengths, fah1 fah2 was crossed with ceramide ...
  • Ebooks (Sách điện tử)

  • Nanofiltration

    • Authors : Farrukh, Muhammad Akhyar (2018)

  • The nanofiltration technique lies between ultrafiltration and reverse osmosis techniques, and it is considered a low-cost process and is capable of removing pesticides, organic matter, desalination of sea water, oil process and pollutants from industrial wastewater. However, the main challenge in implementation of nanofiltration membrane is its ability towards fouling and low performance at high temperature. The use of nanoparticles in the manufacturing of membranes&#x...
  • Journal article

  • SARS-CoV-2 spike variants differ in their allosteric response to linoleic acid

    • Authors : Oliveira, A. Sofia F. (2023)

  • The SARS-CoV-2 spike protein contains a fatty acid binding site, also found in some other coronaviruses (e.g. SARS-CoV), which binds linoleic acid and is functionally important. When occupied by linoleic acid, it reduces infectivity, by ‘locking’ the spike in a less infectious conformation. Here, we use dynamical-nonequilibrium molecular dynamics (D-NEMD) simulations to compare the response of spike variants to linoleic acid removal. These simulations show that&#x...
  • Journal article

  • Acidophilic Micrarchaeon Seems to Maintain a Slightly Alkaline Cytosolic pH

    • Authors : Winkler, Dennis (2021)

  • Despite several discoveries in recent years, the physiology of acidophilic Micrarchaeota 16 remains largely enigmatic. “Candidatus Micrarchaeum harzensis A_DKE”, for example, highly 17 expresses numerous genes encoding hypothetical proteins and their function is difficult to 18 elucidate due to a lacking genetic system. Still, not even the intracellular pH value of A_DKE 19 is known, and heterologous production attempts are generally missing so far. Hence, A_DKE’s...
  • Journal article

  • First direct observation of ‘elongated’ conformational states in α-synuclein upon liquid-liquid phase separation

    • Authors : Ubbiali, Daniele (2023)

  • α-Synuclein (α-syn) is an intrinsically disordered protein (IDP) that undergoes liquid-liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy’s bodies in neurons, which are the hallmark of Parkinson’s Disease (PD). Neurotoxicity precedes the formation of aggregates and is probably related to LLPS of α-syn in the cell. The molecular mechanisms underlying the early stages of LLPS are still elusive. To obtain s...
  • Journal article

  • Uncovering the structural flexibility of SARS-CoV-2 glycoprotein spike variants

    • Authors : Arruda, Hiam R. S. (2023)

  • The severe acute respiratory syndrome CoV-2 rapidly spread worldwide, causing a 43 pandemic. After a period of evolutionary stasis, a set of SARS-CoV-2 mutations has arisen in the 44 spike, the leading glycoprotein at the viral envelope and the primary antigenic candidate for 45 vaccines against the 2019 CoV disease (COVID-19). Here, we present comparative biochemical 46 data of the glycosylated full-length ancestral and D614G spike to...
  • Journal article

  • Mechanistic basis for oxidative stress protection of the human tRNA ligase complex by the oxidoreductase PYROXD1

    • Authors :  Loeff, Luuk (2023)

  • RTCB is the catalytic subunit of the metazoan tRNA ligase complex (tRNA-LC) that plays essential roles in tRNA biogenesis and unfolded protein response. The catalytic center of RTCB contains a conserved cysteine that is susceptible to metal ion-induced oxidative inactivation. The flavin-containing oxidoreductase PYROXD1 preserves the activity of mammalian tRNA-LC in a NAD(P)H-dependent manner, but its protective mechanism remains elusive. Here we report a cryo-EM ...
  • Journal article

  • Molecular insights into the effect of alkanediols on FUS liquid-liquid phase separation

    • Authors : Perdikari, Theodora Myrto (2023)

  • Numerous cell biology studies have used high concentrations of 1,6-hexanediol to dissolve membraneless organelles and disordered protein biomolecular condensates. Yet, little is known about how alkanediols effect liquid-liquid phase separation (LLPS), and why certain alkanediol isomers are more effective. Here, we evaluate the effect of various alkanediols on the archetypal phase separating protein FUS. Low-complexity domain and full-length FUS LLPS is d...
  • Journal article

  • Mass Spectrometry Reveals that Oxysterols are Secreted from Non-Alcoholic Fatty Liver Disease Induced Organoids

    • Authors : Kømurcu, Kristina Sæterdal (2023)

  • Oxysterols are potential biomarkers for liver metabolism that are altered under disease 13 conditions such as non-alcoholic fatty liver disease (NAFLD). We here apply sterolomics to 14 organoids used for disease modeling of NAFLD. Using liquid chromatography-mass spectrometry 15 with on-line sample clean-up and enrichment, we establish that liver organoids produce and 16 secrete oxysterols. We find elevated levels of 26-hydroxycholesterol, an LXR ago...
  • Journal Article

  • Fluorogenic Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide Activated SIRT7

    • Authors : Bolding, Julie E. (2023)

  • The sirtuins are NAD+-dependent lysine deacylases, comprising seven isoforms (SIRT1–7) in humans, which are involved in the regulation of a plethora of biology, including gene expression and metabolism. The sirtuins share a common hydrolytic mechanism but display preferences for different ε-N-acyllysine substrates. SIRT7 deacetylates targets in nuclei and nucleoli but remains one of the lesser studied of the seven isoforms; in part, because of a...