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  • Ebooks (Sách điện tử)

  • Life, Re-Scaled The Biological Imagination in Twenty-First-Century Literature and Performance

    • Authors : Campos, Liliane; Patoine, Pierre-Louis (2022)

  • Looking back at the twentieth century, evolutionary biology and genetics stand out as two immensely influential sciences, whose images and discourses shaped the imagination of life across cultural forms. While Darwin’s ‘plots’, as Gillian Beer noted in 2000, continued to generate productive and conflicting narratives, Darwin’s presence ‘in argument and in popular imagination’ was strengthened by the progressive rise of genetic research.1 The&#...
  • Journal article

  • Stem cell quiescence requires PRC2/PRC1-mediated mitochondrial checkpoint

    • Authors : Ishibashi, JR (2021)

  • Both normal and tumorous stem cells can arrest cell division, avoid apoptosis, and then regenerate lost daughter cells following acute genotoxic insult. This protective, reversible proliferative arrest, known as “quiescence,” is still poorly understood. Here, we show that mTOR-regulated mitophagy is required for radiation insult-induced quiescence in Drosophila germline stem cells (GSCs). In GSCs, depletion of mito-fission (Drp1) or mitophagy �...
  • Journal article

  • HaloTag9: an engineered protein tag to improve fluorophore performance

    • Authors : Frei, Michelle S. (2021)

  • HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a...
  • Journal Article

  • Structure of HK97 small terminase:DNA complex unveils a novel DNA binding mechanism by a circular protein

    • Authors : chechik, Maria (2023)

  • The large group of dsDNA viruses comprising tailed bacteriophages and herpesviruses assemble by packaging their DNA into preformed procapsids. The key component ensuring specific recognition of bacteriophage DNA from the mixture of all the nucleic acids contained in the host cell, and initiating its packaging is the small terminase protein. The nascent genome is usually produced as multiple copies, joined head-to-tail, in long concatemers of dsDN...
  • Journal Article

  • Engineered Antigen-Binding Fragments for Enhanced Crystallisation of Antibody:Antigen Complexes

    • Authors : Bruce, Heather Ann (2023)

  • The atomic-resolution structural information that X-ray crystallography can provide on the binding interface between a Fab and its cognate antigen is highly valuable for understanding the mechanism of interaction. However, many Fab:antigen complexes are recalcitrant to crystallisation, making the endeavour a significant effort with no guarantee of success. Consequently, there have been significant steps taken to increase the likelihood of Fab:antigen complex crystallisation ...
  • Journal Article

  • Algal kainoid synthases exhibit substrate-dependent hydroxylation and cyclization activities

    • Authors : Hopiavuori, Austin R. (2023)

  • FeII/a-ketoglutarate-dependent dioxygenases (Fe/aKG) are a large enzyme family that functionalize C-H bonds on diverse organic substrates. Although Fe/aKG homologs catalyze an array of chemically useful reactions, hydroxylation typically predominates. Microalgal DabC uniquely forms a novel C-C bond to construct the bioactive pyrrolidine ring in domoic acid biosynthesis. However, this kainoid synthase exclusively performs a stereospecific hydroxylation reaction on it...
  • Journal article

  • Structure and function of a Notch-like proteolytic switch domain in the extracellular matrix receptor dystroglycan

    • Authors : Anderson, Michael J.M. (2023)

  • The adhesion receptor dystroglycan provides a critical mechanical link between the extracellular matrix (ECM) and the actin cytoskeleton to help muscle cells withstand contraction and neural cells maintain the blood brain barrier. Disrupting the link is associated cancer and muscular dystrophy. Proteolysis of dystroglycan by matrix metalloproteinases (MMPs) provides a mechanism to break the mechanical anchor and is amplified in several pathogenic states,&...
  • Journal article

  • Screening of candidate host cell membrane proteins involved in SARS-CoV-2 entry

    • Authors : Kotani, Norihiro (2023)

  • Coronavirus disease (COVID-19) represents a real threat to the global population, and understanding the biological features of the causative virus, i.e., severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is imperative for mitigating this threat. Analyses of proteins such as primary receptors and co-receptors (co-factors), which are involved in the entry of SARSCoV- 2 into host cells, will provide important clues to help control the virus. Here,&#...