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  • Journal article

  • Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors

    • Authors : Oliveira, A. Sofia F.; Ibarra, Amaurys Avila; Bermudez, Isabel (2020)

  • The binding of the Y674-R685 loop of the S protein to three nAChRs, namely the human α4β2 and α7 subtypes and the muscle-like αβγδ receptor from Tetronarce californica. Our results indicate that Y674-R685 has affinity for nAChRs and the region responsible for binding contains the PRRA motif, a four-residue insertion not found in other SARS-like coronaviruses. In particular, R682 has a key role in the stabilisation of the complexes as it&#x...
  • Journal article

  • Activation of innate immunity by neutral polysaccharides from broccoli buds

    • Authors : Miyashita, Atsushi (2021)

  • Edible substances that stimulate the innate immune system are good candidates for functional foods to improve human health. We have previously reported that acidic polysaccharides from broccoli extract exhibit immunostimulatory effects, but neutral polysaccharides have been overlooked. In the present study, we found that neutral polysaccharides have significantly stronger (higher specific activity) immunostimulatory activity than acidic polysaccharides. The hot water extract of...
  • Journal Article

  • Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors

    • Authors : Maharana, Jagannath (2023)

  • β-arrestins are multifunctional proteins that are critically involved in regulating spatio26 temporal aspects of GPCR signaling. The interaction of β-arrestins with GPCRs is typically conceptualized in terms of receptor activation and phosphorylation primarily in the carboxyl terminus. Interestingly however, there are several GPCRs that harbor majority of phosphorylation sites in their 3rd intracellular loop (ICL3) instead of carboxyl-terminus but still ...
  • Journal Article

  • Molecular details of the CPSF73-CPSF100 C-terminal heterodimer and interaction with Symplekin

    • Authors : Thore, Stéphane (2023)

  • Eukaryotic pre-mRNA is processed by a large multiprotein complex to accurately cleave the 3’ end, and to catalyze the addition of the poly(A) tail. Within this cleavage and polyadenylation specificity factor (CPSF) machinery, the CPSF73 endonuclease subunit directly contacts both CPSF100 and the scaffold protein Symplekin to form a subcomplex known as the core cleavage complex (CCC) or mammalian cleavage factor (mCF). Here we have taken advantage of ...
  • Journal article

  • Myo-inositol Oxygenase Overexpression Rescues Vitamin C Deficient Arabidopsis (vtc) Mutants

    • Authors : Lucia, M. Acosta-Gamboa (2021)

  • Biosynthesis of L-ascorbate (AsA) in plants is carried out by a complex metabolic network, which involves D-mannose/L-galactose, D-galacturonate, L-gulose, and myo-inositol as main precursors. Arabidopsis lines over-expressing enzymes in the myo-inositol pathway have elevated AsA, accumulate more biomass of both aerial and root tissues, and are tolerant to abiotic stresses as shown by manual and digital phenotyping. We crossed myo-inositol oxygenase (MIOX4) over...
  • Periodicals (Báo – Tạp chí)

  • Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms

    • Authors : Malik1, Asad U. (2020)

  • Much attention has focused on LRRK2, as autosomal dominant missense mutations that enhance its kinase activity cause inherited Parkinson’s disease. LRRK2 regulates biology by phosphorylating a subset of Rab GTPases including Rab8A and Rab10 within its effector binding motif. In this study we explore whether LRRK1, a less studied homologue of LRRK2 that regulates growth factor receptor trafficking and osteoclast biology might also phosphorylate Rab proteins. U...
  • Journal Article

  • Nucleotide-protocell interactions: A reciprocal relationship in prebiotically pertinent environments

    • Authors : deshpande, kshitij (2023)

  • Compartmentalization is a primary attribute of cellular life, and hence, the emergence of compartments is considered a central step in the emergence of the earliest protocells.[1–3] Life is thought to have originated in chemically heterogeneous environments, where different chemical entities would have spontaneously interacted with each other, affecting each other’s properties.[4,5] Fatty acids and nucleotides are found abundantly in extant life and are also considered...
  • Journal Article

  • Impact of combinatorial histone modifications on acetyllysine recognition by the ATAD2 and ATAD2B bromodomains

    • Authors : Phillips, Margaret (2023)

  • The ATPase family AAA+ domain containing 2 (ATAD2) protein, and its paralog ATAD2B, have a C-terminal bromodomain that functions as a ‘reader’ of acetylated lysine residues on histone proteins. Using a structure-function approach, we investigated the ability of the ATAD2 and ATAD2B bromodomains to select acetylated lysine among multiple histone post-translational modifications. Isothermal titration calorimetry experiments revealed that the ATAD2 and ATAD...