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  • Professional Practice Paper

  • Fatty Acyl Availability Modulates Cardiolipin Composition and Alters Mitochondrial Function in HeLa Cells

    • Authors : Oemer, Gregor; Hammel, Forrest A.; Janetzko, John (2020)

  • The molecular assembly of cells depends not only on their balance between anabolism and catabolism, but to a large degree also on the building blocks available in the environment. For cultivated mammalian cells, this is largely determined by the composition of the growth medium used. Here we study the impact of medium lipids on mitochondrial membrane architecture and function by combining LC-MS/MS lipidomics and functional tests with lipid supp...
  • Professional Practice Paper

  • Protein substrates engage the lumen of O-GlcNac transferase’s tetratricopeptide repeat domain in different ways

    • Authors : Joiner, Cassandra M. (2020)

  • Glycosylation of nuclear and cytoplasmic proteins is an essential post-translational modification in mammals. O-GlcNAc transferase (OGT), the sole enzyme responsible for this modification, glycosylates over a thousand unique nuclear and cytoplasmic substrates. How OGT selects its substrates is a fundamental question that must be answered to understand OGT’s unusual biology. OGT contains a long tetratricopeptide repeat (TPR) domain that has been implicated in substrate ...
  • Professional Practice Paper

  • Engineered pH-Sensitive Protein G / IgG Interaction

    • Authors : Jha, Ramesh K. (2020)

  • While natural protein-protein interactions have evolved to be induced by complex stimuli, rational design of interactions that can be switched-on-demand still remain challenging in the protein design world. Here, we demonstrate a computationally redesigned natural interface for improved binding affinity could further be mutated to adopt a pH switchable interaction. The redesigned interface of Protein G-IgG Fc domain, when incorporated with histidine and glutamic acid&#...
  • Periodicals (Báo – Tạp chí)

  • Electromembrane extraction 1 and mass spectrometry for 2 liver organoid drug metabolism studies

    • Authors : Skottvoll, Frøydis Sved (2020)

  • Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME, allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multi-well EME (Para...
  • Ebooks (Sách điện tử)

  • Direct detection of coupled proton and electron transfers in human manganese superoxide dismutase

    • Authors : Azadmanesh, Jahaun (2020)

  • Human manganese superoxide dismutase (MnSOD) is a critical oxidoreductase found in the mitochondrial matrix. Concerted proton and electron transfers (CPETs) are used by the enzyme to rid the mitochondria of O2, a precursor to other harmful reactive oxygen and nitrogen species. The mechanisms of CPET-utilizing enzymes are typically unknown due to the difficulties in detecting the protonation states of specific residues and solvent molecules involved in catalys...
  • Periodicals (Báo – Tạp chí)

  • De novo 3D models of SARS-CoV-2 RNA elements and smallmolecule-binding RNAs to aid drug discovery

    • Authors : Rangan, Ramya (2020)

  • he rapid spread of COVID-19 is motivating development of antivirals targeting conserved SARS-CoV-2 molecular machinery. The SARS-CoV-2 genome includes conserved RNA elements that offer potential small-molecule drug targets, but most of their 3D structures have not been experimentally characterized. Here, we provide a compilation of chemical mapping data from our and other labs, secondary structure models, and 3D model ensembles based on Rosetta’s FARFAR2 algorithm ...
  • Periodicals (Báo – Tạp chí)

  • The RNA-binding protein Nab2 regulates the proteome of the developing Drosophila brain

    • Authors : Corgiat, Edwin B. (2020)

  • The human ZC3H14 gene, which encodes a ubiquitously expressed polyadenosine zinc finger RNA binding protein, is mutated in an inherited form of autosomal recessive, non-syndromic intellectual disability. To gain insight into ZC3H14 neurological functions, we previously developed a Drosophila melanogaster model of ZC3H14 loss by deleting the fly ortholog, Nab2. Studies in this invertebrate model reveal that Nab2 controls final patterns of neuron projection within f...