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  • Periodicals (Báo – Tạp chí)

  • RNA and DNA G-quadruplexes bind to human Dicer and inhibit its activity

    • Authors : Koralewska, Natalia (2020)

  • Guanine (G)-rich single-stranded nucleic acids can adopt G-quadruplex structures. Accumulating evidence indicates that G-quadruplexes serve important regulatory roles in fundamental biological processes such as DNA replication, transcription, and translation, while aberrant G-quadruplex formation is linked to genome instability and cancer. Understanding the biological functions played by G-quadruplexes requires detailed knowledge of their protein interactome. Here, we report that both RNA ...
  • Professional Practice Paper

  • HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase Johannes

    • Authors : Rudolph, Johannes (2020)

  • Poly(ADP-ribose) polymerase 1 (PARP1) is an important player in the response to DNA damage. Recently, histone PARylation factor (HPF1) was shown to be a critical modulator of the activity of PARP1 by facilitating PARylation of histones and redirecting the target amino acid specificity from acidic to serine residues. Here we investigate the mechanism and specific consequences of HPF1-mediated PARylation using nucleosomes as both activators and substrates for&#...
  • Periodicals (Báo – Tạp chí)

  • A clinically-relevant polymorphism in the Na+/taurocholate cotransporting polypeptide (NTCP) occurs at a rheostat position

    • Authors : Strzelecka, Dominika; Smietanski, Miroslaw (2020)

  • Chemical modifications enable preparation of mRNAs with augmented stability and translational activity. In this study, we explored how chemical modifications of 5’,3’-phosphodiester bonds in the mRNA body and polyA tail influence the biological properties of eukaryotic mRNA. To obtain modified and unmodified in vitro transcribed mRNAs, we used ATP and ATP analogues modified at the α-phosphate (containing either O-to-S or O-to-BH3 substitutions) and three different ...
  • Ebooks (Sách điện tử)

  • Earth 2020 An Insider’s Guide to a Rapidly Changing Planet

    • Authors : Tortell, Philippe D. (2020)

  • Such lessons and insights are gathered together in the present collection of essays, which mark the fiftieth anniversary of Earth Day in 2020. The idea for such a collection first came to me in January 2019, just a few months after I had resigned as Director of the Institute for Advanced Studies at the University of British Columbia (UBC). It was a job I had held for the better part of three years, du...
  • Periodicals (Báo – Tạp chí)

  • Amphipathic Fluorescent Dyes for Sensitive and Long-Term Monitoring of Plasma Membranes

    • Authors : Daly, Leonard (2020)

  • Adaption of cells to low oxygen environments is an essential process mediated in part by the Hypoxia Inducible Factors (HIFs). Like other transcription factors, the stability and transcriptional activity of HIFs, and consequently he hypoxic response, are regulated by post-translational modification (PTM) and changes in biomolecular interactions. However, our current understanding of PTM-mediated regulation of HIFs is primarily based on in vitro protein fragment-based s...
  • Periodicals (Báo – Tạp chí)

  • Comparative biochemistry of four polyester (PET) hydrolases

    • Authors : Baath, Jenny Arnling (2020)

  • The potential of bioprocessing in a circular plastic economy has strongly stimulated research in enzymatic egradation of different synthetic resins. Particular interest has been devoted to the commonly used polyester, oly(ethylene terephthalate) (PET), and a number of PET hydrolases have been described. However, a kinetic ramework for comparisons of PET hydrolases (or other plastic degrading enzymes) acting on the insoluble ubstrate, has not been established. Here...
  • Periodicals (Báo – Tạp chí)

  • Molecular Elucidation of Pancreatic Elastase Inhibition by Baicalein

    • Authors : Ghosh, Debajeet (2020)

  • The serine protease, elastase exists in various forms and plays diverse roles in the human body. Pharmacological inhibition of elastase has been investigated for its therapeutic role in managing conditions such as diabetes, pneumonia and arthritis. Sivelestat, a synthetic molecule, is the only elastase inhibitor to have been approved by any major drug regulatory authority (PMDA, in this case) – but still has failed to attain widespread clinical usag...
  • Periodicals (Báo – Tạp chí)

  • Extension of a de novo TIM barrel with a rationally designed secondary structure element

    • Authors : Wiese, Jonas Gregor (2020)

  • To explore opportunities to extend and diversify this scaffold by adding a short de novo helix on top of the barrel. Due to the size of the protein we developed a design pipeline based on computational ab initio folding that solves a less complex sub-problem focused around the helix and its vicinity and adapt it to the entire protein. We provide biochemical characterization and a high-resolution X-ray structure for one variant and...