- Periodicals (Báo – Tạp chí)
Authors : Arturo, Emilia C. (2020) - Phenylalanine hydroxylase (PAH) is an allosteric enzyme responsible for maintaining phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria. Wild type (WT) PAH equilibrates among resting-state RS-PAH) and activated (A-PAH) conformations, whose equilibrium position depends upon allosteric Phe binding to he A-PAH conformation. The RS-PAH conformation of WT rat PAH (rPAH) contains a cation-π sandwich etween Phe80, Arg123, and Arg420, which cannot ex...
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- Journal article
Authors : Chen, Dapeng; Bryden, Wayne A; McLoughlin, Michael (2020) - Characterization of nonvolatile molecules in exhaled breath particles can be used for respiratory disease monitoring and diagnosis. Conventional methods for the collection of nonvolatile molecules in breath heavily rely on the physical properties of exhaled breath particles. Strategies taking advantage of their chemical properties have not yet been explored.
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- Journal article
Authors : Saladi, Shyam M.; Maggiolo, Ailiena O.; Radford, Kate (2020) - Three keys, and have written accompanying software plugins, for structural biologists to create figures truer to the hard-won data and clearer across different modes of color vision and to non-expert readers: Use perceptually uniform colormaps; Consider readers with different modes of color vision; Be explicit about scales and color usage
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- Journal article
Authors : Ng., Wenfa (2020) - Visualization of the reversed phase HPLC chromatogram at 194 nm revealed broad peaks and lack of distinct peaks indicative of fractionation of LB Lennox medium by the chromatography method. More importantly, complex ensemble of different components in the growth medium present a significant separation challenge that precludes separation or fractionation by modern liquid chromatography methods. Overall, combinatorial use of both hydrophobic and hydrophilic mobile phase&...
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- Journal article
Authors : Junglas, Benedikt; Axt, Amelie; Siebenaller, Carmen (2020) - IM30 is involved in thylakoid membrane biogenesis and/or maintenance, where it either stabilizes membranes and/or triggers membrane-fusion processes. These apparently contradicting processes have to be tightly controlled and separated spatiotemporally in chloroplasts and cyanobacteria. The latter process depends on Mg2+-binding to IM30; yet, it still is unclear how Mg2+-loaded IM30 interacts with membranes and promotes membrane fusion. Here we show that interaction of&...
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- Journal article
Authors : Kmiecik, Szymon W.; Drzewicka, Katarzyna; Melchior, Frauke (2020) - According to cell biological and proteomics data, Hsf1 is also modified by SUMO (small ubiquitin-like modifier) at several sites. How SUMOylation affects Hsf1 activity at a molecular level is still unclear. Here, we analyzed Hsf1 SUMOylation in vitro with purified components to address questions that could not be answered in cell culture models. In vitro Hsf1 is primarily conjugated at lysine 298 with a single SUMO, though we did detect lo...
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- Periodicals (Báo – Tạp chí)
Authors : Takeuchi, Koh; Ikeda, Yoshiki; Harada, Ayaka (2020) - Most kinases function with ATP. However, contrary to the prevailing dogma, phosphatidylinositol 5-phosphate 4-kinase β (PI5P4Kβ) utilizes GTP as a primary phosphate donor with a unique binding mode for GTP. Although PI5P4Kβ is evolved from a primordial ATP-utilizing enzyme, PI4P5K, how PI5P4Kβ evolutionarily acquired the GTP preference to function as a cellular GTP sensor remains unclear
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- Journal article
Authors : Wybenga-Groot, Leanne E.; McGlade, C. Jane (2020) - The Src-like adaptor proteins (SLAP/SLAP2) bind to CBL E3 ubiquitin ligase to downregulate antigen, cytokine, and tyrosine kinase receptor signaling. In contrast to phospho-tyrosine dependent binding of CBL substrates through its tyrosine kinase binding domain (TKBD), CBL TKBD associates with the C-terminal tail of SLAP2 in a phospho-independent manner. To understand the distinct nature of this interaction, a purification protocol for SLAP2 in complex with CB...
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- Periodicals (Báo – Tạp chí)
Authors : Malik1, Asad U. (2020) - Much attention has focused on LRRK2, as autosomal dominant missense mutations that enhance its kinase activity cause inherited Parkinson’s disease. LRRK2 regulates biology by phosphorylating a subset of Rab GTPases including Rab8A and Rab10 within its effector binding motif. In this study we explore whether LRRK1, a less studied homologue of LRRK2 that regulates growth factor receptor trafficking and osteoclast biology might also phosphorylate Rab proteins. U...
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- Ebooks (Sách điện tử)
Authors : NagaratnamP, Nirupa (2020) - Proteases can play essential roles in severe human pathology, ranging from degenerative and inflammatory illnesses to infectious diseases, with some, such as Taspase1, involved in growth and progression of tumors at primary and metastatic sites. Taspase1 is a N-terminal nucleophile (Ntn)-hydrolase overexpressed in primary human cancers, coordinating cancer 3Tcell proliferation, invasion, and metastasis. L3Toss of Taspase1 activity disrupts proliferation of human cancer cells...
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