- Journal article
Authors : Schäfer, an-Hannes (2023) - Sphingolipids are structural membrane components that also function in cellular stress 32 responses. The serine palmitoyl-transferase (SPT) catalyzes the rate limiting step in 33 sphingolipid biogenesis. Its activity is tightly regulated through multiple binding part-34 ners, including Tsc3, Orm proteins, ceramides, and the phosphatidylinositol-4-phos-35 phate (PI4P) phosphatase Sac1. The structural organization and regulatory mecha-36 nisms of this complex are not yet understood.
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- Ebooks (Sách điện tử)
Authors : Madabushi, Sowmya (2023) - Puromycin-sensitive aminopeptidase (E.C. 3.4.11.14, UniProt P55786), a zinc
6 metallopeptidase belonging to the M1 family, degrades a number of bioactive peptides as
7 well as peptides released from the proteasome, including polyglutamine. We report the
8 crystal structure of PSA at 2.3 Ǻ. Overall, the enzyme adopts a V-shaped architecture
9 with four domains characteristic of the M1 family aminopeptidases, but it is in a less
10...
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- Journal article
Authors : Richards, Logan S. (2023) - Amyloid diseases are linked to the formation and persistence of large, multimeric structures in
various tissues. Amyloid fibrils are characterized by a cross-beta scaffold in which identical
protein molecules mate tightly as beta-strands to form a long, unbranched fibril1,2. Before
amyloid-forming proteins assemble into fibrils, they can display a globular fold, requiring partial
or total unfolding to convert into an amyloid state3,4. Debilitating ne...
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- Journal article
Authors : Banerjee, Sayanika (2023) - Ufmylation, a protein modification by Ubiquitin-like (UBL) protein UFM1, plays a crucial
role in several cellular processes including DNA damage response, protein translation and
ER homeostasis. To date, little is known how the enzymes responsible for this modification
coordinate their action. Here we have studied the details of UFL1 (E3) activity, its binding
to UFC1 (E2), and its relation to UBA5 (E1), using a combination of structur...
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- Journal article
Authors : McCorvie, Thomas J. (2023) - Cystathionine beta-synthase (CBS) is an essential metabolic enzyme across all
domains of life involved in the production of glutathione, cysteine, and hydrogen
sulphide1-4. Human CBS appends to its conserved catalytic domain a regulatory domain
that modulates activity by S-adenosyl-L-methionine (SAM) and promotes
oligomerization5-12, however the molecular basis is unknown. Here we show using
cryo-electron microscopy that full-length human CBS in the basa...
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- Journal article
Authors : Shafiei, Alaleh (2023) - Frataxin is a 23 KDa mitochondrial iron-binding protein that is involved in
biogenesis of iron sulfur clusters. A deficiency in frataxin leads to Friedreich's ataxia,
a progressive neurodegenerative disorder. The bacterial ortholog of eukaryotic
mitochondrial frataxin, CyaY, is thought to play a role in iron sulfur cluster
assembly as an iron supplier, making it an important target for study. Here, we
present the first ambient tem...
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- Journal article
Authors : Bickers, Sarah C. (2023) - ATP binding cassette (ABC) proteins generally couple ATP hydrolysis to the active
20 transport of solutes across cellular membranes. All ABC proteins contain a core structure of two
21 transmembrane domains (TMD1, TMD2) and two nucleotide binding domains (NBD1, NBD2),
22 and many ABC proteins contain additional domains. Some members of the C subfamily of ABC
23 (ABCC) proteins, such as the multidrug resistant protein 1 (MRP1), con...
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- Journal Article
Authors : Ikei, Mai (2023) - From bacteria to eukaryotes, sulfur is a vital element for cellular activities. For example, sulfur34
containing biomolecules, such as L-cysteine, L-methionine, thiamine, glutathione, and biotin, play a
35 variety of essential roles in cells (1). Bacteria and plants can utilize L-cysteine as a source of sulfate,
36 but they also have sulfur assimilation pathways to synthesize L-cysteine from inorganic sulfur
37 compounds. In bacteria, L-cysteine...
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- Journal Article
Authors : Jojoa-Cruz, Sebastian (2023) - Members of the OSCA/TMEM63 are mechanically activated ion channels and structures
of some OSCA members have revealed the architecture of these channels and
structural features that are potentially involved in mechanosensation. However, these
structures are all in a similar state and information about the motion of different
elements of the structure is limited, preventing a deeper understanding of how these
channels work. Here, we use...
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- Journal article
Authors : Markus, Linda M. D. (2023) - Cyanophycin is a natural polymer composed of a poly-aspartate backbone with arginine attached
to each of the aspartate sidechains. Produced by a wide range of bacteria, which mainly use it as
a store of fixed nitrogen, it has many promising industrial applications. Cyanophycin can be
synthesized from the amino acids Asp and Arg by the widespread cyanophycin synthetase 1
(CphA1), or from the dipeptide β-Asp-Arg by the cyanobacteria...
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