- Journal article
Authors : Arruda, Hiam R. S. (2023) - The severe acute respiratory syndrome CoV-2 rapidly spread worldwide, causing a
43 pandemic. After a period of evolutionary stasis, a set of SARS-CoV-2 mutations has arisen in the
44 spike, the leading glycoprotein at the viral envelope and the primary antigenic candidate for
45 vaccines against the 2019 CoV disease (COVID-19). Here, we present comparative biochemical
46 data of the glycosylated full-length ancestral and D614G spike to...
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- Journal article
Authors : Fürsch, Julius (2021) - Small heat-shock proteins (sHSP) are important members of the cellular stress response
in all species. Their best described function is the binding of early unfolding states and
the resulting prevention of protein aggregation. Most sHSPs exist as oligomers but vary
in size and subunit organization. Many sHSPs exist as a polydisperse composition of
oligomers which undergoes changes in subunit composition, folding status and rel...
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- Journal article
Authors : Mann, Mandeep K (2021) - USP5 is a deubiquitinase that has been implicated in a range of diseases, including cancer, but no USP5-
targeting chemical probe has been reported to date. Here, we present the progression of a chemical series
that occupies the C-terminal ubiquitin-binding site of a poorly characterized zinc-finger ubiquitin binding
domain (ZnF-UBD) of USP5 and allosterically inhibits the catalytic activity of the enzyme. Systematic
exploration of&...
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- Journal article
Authors : Nikolaivits, Efstratios (2021) - Polyphenol oxidases (PPOs) are an industrially relevant family of enzymes, being involved in the post-harvest browning of fruits and vegetables, as well as in human melanogenesis. Their involvement lies in their ability to oxidize phenolic or polyphenolic compounds, that subsequently form pigments. PPO family includes tyrosinases and catechol oxidases, which in spite of their high structural similarity, exhibit different catalytic activities. Long-standing research efforts&...
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- Journal Article
Authors : Mikhael, Abanoub (2023) - Lipopolysaccharide (LPS) is a hallmark virulence factor of Gram-negative bacteria. It is a complex, structurally heterogeneous mixture due to variations in number, type, and position of its simplest units: fatty acids and monosaccharides. Thus, LPS structural characterization by traditional mass spectrometry (MS) methods is challenging. Here, we describe the benefits of field asymmetric ion mobility spectrometry (FAIMS) for analysis of intact R-type lipopolysaccharide compl...
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- Journal Article
Authors : Que, Nanette L.S. (2023) - Grp94 is the endoplasmic reticulum paralog of the hsp90 family of chaperones, which have
been targeted for therapeutic intervention via their highly conserved ATP binding sites. The
design of paralog-selective inhibitors relies on understanding the structural elements that
mediate each paralog’s response to inhibitor binding. Here, we determined the structures of
Grp94 and Hsp90 in complex with the Grp94-selective inhibitor PU-H36, and of Grp94 ...
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- Journal Article
Authors : Covill-Cooke, Christian (2023) - Miro proteins are universally conserved mitochondrial calcium-binding GTPases that regulate a multitude of mitochondrial processes, including transport, clearance and lipid trafficking. Miro binds a variety of client proteins involved in these functions. How this binding is operated at the molecular level and whether and how it is important for mitochondrial health, however, remains unknown. Here, we show that known Miro clients all use a similar short motif&...
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- Journal Article
Authors : Grassetti, andrew V (2023) - The application of support layers, such as graphene, to cryo-electron microscopy grids can increase the density
of particles imaged, limit particle interactions with the air-water interface, reduce the extent of beam-induced
motion, and, in some instances, improve the distribution of particle orientations. This paper describes a robust
protocol for coating cryo-EM grids with a monolayer of graphene for improved cryo-sample preparation. Single particle cryo...
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- Journal article
Authors : Moreira, Mariana H. (2021) - Defensins are small proteins, usually ranging from 4 to 6 kDa, amphipathic, disulfide rich, and with a small or even absent hydrophobic core. Since a hydrophobic core is
generally found in globular proteins that fold in an aqueous solvent, the peculiar fold of
defensins can challenge tertiary protein structure predictors. We performed a PDB-wide
survey of small proteins (4-6 kDa) to understand the similarities of defensins ...
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- Journal article
Authors : Sarnowski, Chris P. (2023) - Cross-linking coupled with mass spectrometry is an increasingly popular methodology for elucidating structural information from biological complexes. Whilst protein-protein cross-linking workflows are widely used and well characterised, adoption of protein-RNA cross-linking workflows for structural studies is less widespread, and data produced from such experiments remains less well understood. The cross-linking of stable isotope labelled RNA coupled to mass spectrometry (CLIR-MS) workflo...
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