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  • Journal article

  • Structural and Preliminary Biochemical Characterization of MppQ, a PLP-Dependent Aminotransferase from Streptomyces hygroscopicus

    • Authors : Vuksanovic, Nemanja (2023)

  • MppQ is an enzyme of unknown function from Streptomyces hygroscopicus that is involved in the biosynthesis of the nonproteinogenic amino acid L-enduracididine (L-End). Since L-End is a component of several peptides showing high activity against methicillin-resistant Staphylococcus aureus (MRSA), a complete understanding of its biosynthetic pathway is of utmost importance for developing chemoenzymatic routes for syntheses of novel antibiotics. In this work, we report hi...
  • Journal Article

  • Cardiomyopathy-Associated Variants Alter the Structure and Function of the α-Actinin-2 Actin-Binding Domain

    • Authors : E Atang, Alexandra (2023)

  • Hypertrophic cardiomyopathy (HCM), dilated cardiomyopathy (DCM), and restrictive cardiomyopathy (RCM) are characterized by thickening, thinning, or stiffening, respectively, of the ventricular myocardium, resulting in diastolic or systolic dysfunction that can lead to heart failure and sudden cardiac death. Recently, variants in the ACTN2 gene, encoding the protein α-actinin-2, have been reported in HCM, DCM, and RCM patients. However, functional data supporting the pathogenicit...
  • Ebooks (Sách điện tử)

  • Structural Characterization of the Full-length Hantaan Virus Polymerase

    • Authors : keown, Jeremy R (2023)

  • Hantaviridae are a family of segmented negative-sense RNA viruses that contains important human and animal pathogens including Andes orthohantavirus and Hantaan orthohantavirus. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome in the cytoplasm of infected cells. Here we establish the expression and purification of the polymerase from the Old World Hantaan orthohantavirus and characterise the structure&#...
  • Industry article

  • Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease

    • Authors : gerber, janina Lara (2023)

  • RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex. RNA ligation by the tRNA-ligase complex requires GTP-dependent activation of RTCB. This active site guanylylation reaction relies on the activation factor Archease. The mechanistic interplay between both proteins has remained unknown. Here, we report a&...
  • Journal article

  • Structural insights into the organization and channel properties of human Pannexin isoforms 1 and 3

    • Authors : Hussain, Nazia (2023)

  • Pannexins are single-membrane large-pore ion channels that release ATP upon activation. Three isoforms of pannexins, 1, 2, and 3, perform diverse cellular roles, including inflammation, differentiation, neuropathic pain, and ATP release. In this study, we report the cryoEM structure of pannexin 3 at 3.9 Å and characterize the structural differences with pannexin isoforms 1 and 2. We observe the organization of the Pannexin 3 vestibule into&...
  • Journal article

  • Sucrose phosphorylase from Alteromonas mediterranea: structural insight into the regioselective α-glucosylation of (+)-catechin

    • Authors : Goux, Marine  (2023)

  • Flavonoids glycosylation at different positions is paramount to solubility and modulation of bioactivities. Sucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer glucose from sucrose, the donor substrate, onto polyphenols to form glycoconjugates. Here, we report for the first time the structural and enzymatic properties of sucrose phosphorylase from the marine bacteria Alteromonas mediterranea (AmSP). We characterized&#...
  • Journal Article

  • Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

    • Authors : Yadav, Manish K. (2023)

  • The Hydroxycarboxylic acid receptor 2 (HCA2), also known as the niacin receptor or GPR109A, is a prototypical G protein-coupled receptor that plays a central role in the inhibition of lipolytic and atherogenic activities in our body. Interestingly, GPR109A activation also results in vasodilation that is linked to the side-effect of flushing associated with dyslipidemia drugs such as niacin. This receptor continues to be a key target for developing n...
  • Journal article

  • Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation

    • Authors : Choi, Yong Mi (2023)

  • Lipid droplets (LDs) are dynamic organelles that contain an oil core mainly composed of 36 triglycerides (TAG) that is surrounded by a phospholipid monolayer and LD-associated proteins 37 called perilipins (PLINs). During LD biogenesis, perilipin 3 (PLIN3) is recruited to nascent LDs as 38 they emerge from the endoplasmic reticulum. Here, we analyzed how lipid composition affects 39 PLIN3 recruitment to membrane bilayers and LDs, and t...