Tìm kiếm nâng cao

Current filters:
Current filters:

Discover

Search Results

Item hits:
  • Journal article

  • Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

    • Authors : Birke, Ramona (2021)

  • Sulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is validated in live cell imaging experiments in transfected HEK cells and neurons derived from induced pluripotent stem cells (iPSCs). Lastly, we show that&...
  • Journal article

  • Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro

    • Authors : Trainor, Brandon M. (2021)

  • The conventional view regarding regulation of gene expression is based on transcription control. However, a growing number of recent studies has revealed the important additional impact of translational regulation. Eukaryotic translational machinery appears to be capable of reprogramming mRNA translation to generate proteins required to maintain a healthy cellular proteostasis under particular physiological conditions or to adapt to stress. Altho...
  • Journal article

  • Stability of ligand-induced protein conformation influences affinity in maltose-binding protein

    • Authors : Noort, Marco van den (2021)

  • Our understanding of what determines ligand affinity of proteins is poor, even with highresolution structures available. Both the non-covalent ligand-protein interactions and the relative free energies of available conformations contribute to the affinity of a protein for a ligand. Distant, non-binding site residues can influence the ligand affinity by altering the free energy difference between a ligand-free and ligand-bound conformation. Our hypothesis isthat...
  • Journal article

  • TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

    • Authors : Dhenin, Jonathan (2021)

  • Top-down proteomics (TDP) is a powerful technology allowing the char acterization of proteins at the proteoform level using high-resolution tan dem mass spectrometry (MS/MS). Proteoforms correspond to the different forms of a protein arising from all combinatorial sources of variation from a single gene (including combinations of genetic variation, alternative splicing, and post-translational modifications)(Smith and Kelleher, 2018). The complete...
  • Journal article

  • Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activity

    • Authors : Park, Sang Ho (2021)

  • SARS-CoV-2 is the novel coronavirus that is the causative agent of COVID-19, a sometimes-lethal respiratory infection responsible for a world-wide pandemic. The envelope (E) protein, one of four structural proteins encoded in the viral genome, is a 75-residue integral membrane protein whose transmembrane domain exhibits ion channel activity and whose cytoplasmic domain participates in protein-protein interactions. These activities contribute to several aspects ...
  • Journal article

  • Tannic Acid Inhibits α-Synuclein Amyloid Fibril Formation via Binding to the Monomer N-terminal Domain

    • Authors : Stoeber, Jonathan (2021)

  • α-Synuclein (αS) is an intrinsically disordered protein (IDP) that aggregates into amyloid fibrils during the progression of Parkinson’s Disease and other synucleinopathies. The N-terminal domain (residues 1-60) is now understood to play a critical role in the initial nucleation of aggregation, as well as a pivotal role in the monomer-fibril interaction underlying amyloid seeding. Here we report on the interaction between αS and&#x...
  • Journal article

  • The role of glutathione in periplasmic redox homeostasis and oxidative protein folding in Escherichia coli

    • Authors : Knoke, Lisa R. (2023)

  • The thiol redox balance in the periplasm of E. coli depends on the DsbA/B pair for oxidative 14 power and the DsbC/D system as its complement for isomerization of non-native disulfides. 15 While the standard redox potentials of those systems are known, the in vivo redox potential 16 imposed onto protein thiol disulfide pairs in the periplasm remains unknown. Here, we used 17 genetically encoded redox probes (roGFP2 ...
  • Journal article

  • Torsion angles to map and visualize the conformational space of a protein

    • Authors : Ginn, Helen Mary (2023)

  • Present understanding of protein structure dynamics trails behind that of static structures. A torsionangle based approach, called representation of protein entities (RoPE), derives an interpretable conformational space which correlates with data collection temperature, resolution and reaction coordinate. For more complex systems, atomic coordinates fail to separate functional conformational states, which are still preserved by torsion angle-derived space. This indicates...