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  • Journal Article

  • Authors : Davidsen, Kristian (2023)

  • Current methods to quantify the fraction of aminoacylated tRNAs, also known as the 8 tRNA charge, are limited by issues with either low throughput, precision, and/or accuracy. Here, we 9 present an optimized charge tRNA-Seq method that combines previous developments with newly 10 described approaches to establish a protocol for precise and accurate tRNA charge measurements. 11 We verify that this protocol provides robust quantification ...